Protective effect of rutin against diabetes-associated cognitive decline in rats.
Pak J Pharm Sci
; 35(3): 769-775, 2022 May.
Article
em En
| MEDLINE
| ID: mdl-35791475
ABSTRACT
Diabetes is a well-known risk factor for cognitive deficit. Rutin (RUT) possesses diverse pharmacological activities and is widely used in diabetic complication. The aim of this study is to assess the improvement of RUT on diabetes-associated cognitive decline (DACD). In our study, Morris water maze was examined to estimate cognitive function. In hippocampus tissue, spectrophotometer was performed to evaluate super oxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), malondialdehyde (MDA), acetyl cholinesterase (AChE) and choline acetyl transferase (ChAT) levels. Quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) were utilized to analyze Tumor necrosis factor-a (TNF-a) and interleukin-1ß (IL-1ß) contents. Western blot was used to detect the protein expressions of brain derived neurotrophic factor (BDNF), glial fibrillary acidic protein (GFAP), nuclear factor erythroid-2-related factor-2 (Nrf-2) and heme oxygenase-1 (HO-1). Our data revealed that RUT markedly improved learning and memory capacities in Morris water maze test. In hippocampus, RUT markedly inhibited AChE, GFAP MDA, TNF-a and IL-1ß levels and augmented ChAT and BDNF, SOD, CAT, GSH, Nrf-2 and HO-1 levels. In conclusion, RUT may be involved in protection efficacy against STZ-induced cognitive deficits via improvement of oxidative stress, inflammatory response and Nrf-2/HO-1 pathway.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Complicações do Diabetes
/
Diabetes Mellitus
/
Disfunção Cognitiva
Tipo de estudo:
Etiology_studies
/
Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
Pak J Pharm Sci
Assunto da revista:
FARMACIA
/
FARMACOLOGIA
/
QUIMICA
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
China