Engineering a Hierarchical Biphasic Gel for Subcutaneous Vascularization.
Adv Healthc Mater
; 11(19): e2200922, 2022 10.
Article
em En
| MEDLINE
| ID: mdl-35894816
ABSTRACT
Implanted cell-containing grafts require a robust and functional vasculature to supply oxygen and nutrients, as well as clear metabolic waste products. However, it remains challenging to fabricate tunable, vascular-promoting scaffolds without incorporating additional biologics. Here, a biphasic gel consisting of a highly porous aerogel and a degradable fibrin hydrogel for inducing vascularization is presented. The highly porous (>90%) and stable aerogel is assembled from short microfibers by being dispersed in an aqueous solution that can be 3D printed into various configurations. The biphasic gel demonstrates good compression-resistance 70.30% Young's modulus is recovered over 20 cycles of 65% compression under water. Furthermore, it is confirmed that tissue cells and blood vessels can penetrate a thick (≈3 mm) biphasic gel in the subcutaneous space of mice. Finally, the biphasic gel doubles the vascular ingrowth compared to a composite of a commercial surgical polyester felt and a fibrin hydrogel upon subcutaneous implantation in mice after 4 weeks. The design of this biphasic gel may advance the development of vascularized scaffolds.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Produtos Biológicos
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Neovascularização Fisiológica
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Hidrogéis
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Alicerces Teciduais
Limite:
Animals
Idioma:
En
Revista:
Adv Healthc Mater
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Estados Unidos