TNK2/ACK1-mediated phosphorylation of ATP5F1A (ATP synthase F1 subunit alpha) selectively augments survival of prostate cancer while engendering mitochondrial vulnerability.
Autophagy
; 19(3): 1000-1025, 2023 03.
Article
em En
| MEDLINE
| ID: mdl-35895804
ABSTRACT
The challenge of rapid macromolecular synthesis enforces the energy-hungry cancer cell mitochondria to switch their metabolic phenotypes, accomplished by activation of oncogenic tyrosine kinases. Precisely how kinase activity is directly exploited by cancer cell mitochondria to meet high-energy demand, remains to be deciphered. Here we show that a non-receptor tyrosine kinase, TNK2/ACK1 (tyrosine kinase non receptor 2), phosphorylated ATP5F1A (ATP synthase F1 subunit alpha) at Tyr243 and Tyr246 (Tyr200 and 203 in the mature protein, respectively) that not only increased the stability of complex V, but also increased mitochondrial energy output in cancer cells. Further, phospho-ATP5F1A (p-Y-ATP5F1A) prevented its binding to its physiological inhibitor, ATP5IF1 (ATP synthase inhibitory factor subunit 1), causing sustained mitochondrial activity to promote cancer cell growth. TNK2 inhibitor, (R)-9b reversed this process and induced mitophagy-based autophagy to mitigate prostate tumor growth while sparing normal prostate cells. Further, depletion of p-Y-ATP5F1A was needed for (R)-9b-mediated mitophagic response and tumor growth. Moreover, Tnk2 transgenic mice displayed increased p-Y-ATP5F1A and loss of mitophagy and exhibited formation of prostatic intraepithelial neoplasia (PINs). Consistent with these data, a marked increase in p-Y-ATP5F1A was seen as prostate cancer progressed to the malignant stage. Overall, this study uncovered the molecular intricacy of tyrosine kinase-mediated mitochondrial energy regulation as a distinct cancer cell mitochondrial vulnerability and provided evidence that TNK2 inhibitors can act as "mitocans" to induce cancer-specific mitophagy.Abbreviations ATP5F1A ATP synthase F1 subunit alpha; ATP5IF1 ATP synthase inhibitory factor subunit 1; CRPC castration-resistant prostate cancer; DNM1L dynamin 1 like; MAP1LC3B/LC3B microtubule associated protein 1 light chain 3 beta; Mdivi-1 mitochondrial division inhibitor 1; Mut-ATP5F1A Y243,246A mutant of ATP5F1A; OXPHOS oxidative phosphorylation; PC prostate cancer; PINK1 PTEN induced kinase 1; p-Y-ATP5F1A phosphorylated tyrosine 243 and 246 on ATP5F1A; TNK2/ACK1 tyrosine kinase non receptor 2; Ub ubiquitin; WT wild type.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Próstata
/
Autofagia
Limite:
Animals
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Humans
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Male
Idioma:
En
Revista:
Autophagy
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Estados Unidos