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Protein Condensate Formation via Controlled Multimerization of Intrinsically Disordered Sequences.
Garabedian, Mikael V; Su, Zhihui; Dabdoub, Jorge; Tong, Michelle; Deiters, Alexander; Hammer, Daniel A; Good, Matthew C.
Afiliação
  • Garabedian MV; Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.
  • Su Z; Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.
  • Dabdoub J; Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.
  • Tong M; Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.
  • Deiters A; Department of Chemistry, University of Pittsburgh, Philadelphia, Pennsylvania 15260, United States.
  • Hammer DA; Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.
  • Good MC; Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.
Biochemistry ; 61(22): 2470-2481, 2022 11 15.
Article em En | MEDLINE | ID: mdl-35918061
ABSTRACT
Many proteins harboring low complexity or intrinsically disordered sequences (IDRs) are capable of undergoing liquid-liquid phase separation to form mesoscale condensates that function as biochemical niches with the ability to concentrate or sequester macromolecules and regulate cellular activity. Engineered disordered proteins have been used to generate programmable synthetic membraneless organelles in cells. Phase separation is governed by the strength of interactions among polypeptides with multivalency enhancing phase separation at lower concentrations. Previously, we and others demonstrated enzymatic control of IDR valency from multivalent precursors to dissolve condensed phases. Here, we develop noncovalent strategies to multimerize an individual IDR, the RGG domain of LAF-1, using protein interaction domains to regulate condensate formation in vitro and in living cells. First, we characterize modular dimerization of RGG domains at either terminus using cognate high-affinity coiled-coil pairs to form stable condensates in vitro. Second, we demonstrate temporal control over phase separation of RGG domains fused to FRB and FKBP in the presence of dimerizer. Further, using a photocaged dimerizer, we achieve optically induced condensation both in cell-sized emulsions and within live cells. Collectively, these modular tools allow multiple strategies to promote phase separation of a common core IDR for tunable control of condensate assembly.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fenômenos Bioquímicos / Proteínas Intrinsicamente Desordenadas Idioma: En Revista: Biochemistry Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fenômenos Bioquímicos / Proteínas Intrinsicamente Desordenadas Idioma: En Revista: Biochemistry Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos