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Nine out of ten samples were mistakenly switched by The Orang-utan Genome Consortium.
Banes, Graham L; Fountain, Emily D; Karklus, Alyssa; Fulton, Robert S; Antonacci-Fulton, Lucinda; Nelson, Joanne O.
Afiliação
  • Banes GL; Wisconsin National Primate Research Center, University of Wisconsin-Madison, 1220 Capitol Court, Madison, WI, 53715, USA. graham.banes@cantab.net.
  • Fountain ED; School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Drive, Madison, WI, 53706, USA. graham.banes@cantab.net.
  • Karklus A; The Orang-utan Conservation Genetics Project, Madison, WI, 53715, USA. graham.banes@cantab.net.
  • Fulton RS; Wisconsin National Primate Research Center, University of Wisconsin-Madison, 1220 Capitol Court, Madison, WI, 53715, USA.
  • Antonacci-Fulton L; The Orang-utan Conservation Genetics Project, Madison, WI, 53715, USA.
  • Nelson JO; School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Drive, Madison, WI, 53706, USA.
Sci Data ; 9(1): 485, 2022 08 12.
Article em En | MEDLINE | ID: mdl-35961988
The Sumatran orang-utan (Pongo abelii) reference genome was first published in 2011, in conjunction with ten re-sequenced genomes from unrelated wild-caught individuals. Together, these published data have been utilized in almost all great ape genomic studies, plus in much broader comparative genomic research. Here, we report that the original sequencing Consortium inadvertently switched nine of the ten samples and/or resulting re-sequenced genomes, erroneously attributing eight of these to the wrong source individuals. Among them is a genome from the recently identified Tapanuli (P. tapanuliensis) species: thus, this genome was sequenced and published a full six years prior to the species' description. Sex was wrongly assigned to five known individuals; the numbers in one sample identifier were swapped; and the identifier for another sample most closely resembles that of a sample from another individual entirely. These errors have been reproduced in countless subsequent manuscripts, with noted implications for studies reliant on data from known individuals.

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Sci Data Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Sci Data Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Reino Unido