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Estrogenic in vitro evaluation of zearalenone and its phase I and II metabolites in combination with soy isoflavones.
Grgic, Dino; Betschler, Andrea; Früholz, Rebeka; Novak, Barbara; Varga, Elisabeth; Marko, Doris.
Afiliação
  • Grgic D; Department of Food Chemistry and Toxicology, Faculty of Chemistry, University of Vienna, Währinger Str. 38-40, 1090, Vienna, Austria.
  • Betschler A; Doctoral School in Chemistry, University of Vienna, Währinger Str. 38-40, 1090, Vienna, Austria.
  • Früholz R; Department of Food Chemistry and Toxicology, Faculty of Chemistry, University of Vienna, Währinger Str. 38-40, 1090, Vienna, Austria.
  • Novak B; Department of Food Chemistry and Toxicology, Faculty of Chemistry, University of Vienna, Währinger Str. 38-40, 1090, Vienna, Austria.
  • Varga E; DSM, BIOMIN Research Center, Technopark 1, 3430, Tulln, Austria.
  • Marko D; Department of Food Chemistry and Toxicology, Faculty of Chemistry, University of Vienna, Währinger Str. 38-40, 1090, Vienna, Austria. elisabeth.varga@univie.ac.at.
Arch Toxicol ; 96(12): 3385-3402, 2022 12.
Article em En | MEDLINE | ID: mdl-35986755
Humans and animals are exposed to multiple substances in their food and feed that might have a negative health impact. Among these substances, the Fusarium mycoestrogen zearalenone (ZEN) and its metabolites α-zearalenol (α-ZEL) and α-zearalanol (α-ZAL) are known to possess endocrine disruptive properties. In a mixed diet or especially animal feed, these potential contaminants might be ingested together with naturally occurring phytoestrogens such as soy isoflavones. So far, risk assessment of potential endocrine disruptors is usually based on adverse effects of single compounds whereas studies investigating combinatorial effects are scarce. In the present study, we investigated the estrogenic potential of mycoestrogens and the isoflavones genistein (GEN), daidzein (DAI) and glycitein (GLY) as well as equol (EQ), the gut microbial metabolite of DAI, in vitro alone or in combination, using the alkaline phosphatase (ALP) assay in Ishikawa cells. In the case of mycoestrogens, the tested concentration range included 0.001 to 10 nM with multiplication steps of 10 in between, while for the isoflavones 1000 times higher concentrations were investigated. For the individual substances the following order of estrogenicity was obtained: α-ZEL > α-ZAL > ZEN > GEN > EQ > DAI > GLY. Most combinations of isoflavones with mycoestrogens enhanced the estrogenic response in the investigated concentrations. Especially lower concentrations of ZEN, α-ZEL and α-ZAL (0.001-0.01 nM) in combination with low concentrations of GEN, DAI and EQ (0.001-0.1 µM) strongly increased the estrogenic response compared to the single substances.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Zearalenona / Zeranol / Disruptores Endócrinos / Isoflavonas Tipo de estudo: Risk_factors_studies Limite: Animals / Humans Idioma: En Revista: Arch Toxicol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Áustria País de publicação: Alemanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Zearalenona / Zeranol / Disruptores Endócrinos / Isoflavonas Tipo de estudo: Risk_factors_studies Limite: Animals / Humans Idioma: En Revista: Arch Toxicol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Áustria País de publicação: Alemanha