Ultrasensitive and Selective Detection of Glutathione by Ammonium Carbamate-Gold Platinum Nanoparticles-Based Electrochemical Sensor.
Life (Basel)
; 12(8)2022 Jul 28.
Article
em En
| MEDLINE
| ID: mdl-36013320
ABSTRACT
Determining the concentration of glutathione is crucial for developing workable medical diagnostic strategies. In this paper, we developed an electrochemical sensor by electrodepositing amino-based reactive groups and gold-platinum nanomaterials on the surface of glassy carbon electrode successively. The sensor was characterized by cyclic voltammetry (CV), field emission scanning electron microscope (FESEM), energy dispersive X-ray spectroscopy (EDX), and electrochemical impedance spectra (EIS). Results showed that Au@Pt nanoparticles with the size of 20-40 nm were presented on the surface of electrode. The sensor exhibits excellent electrocatalytic oxidation towards glutathione. Based on this, we devised an electrochemical biosensor for rapid and sensitive detection of glutathione. After optimizing experimental and operational conditions, a linear response for the concentration of GSH, in the range of 0.1-11 µmol/L, with low detection and quantification limits of 0.051 µM (S/N = 3), were obtained. The sensor also exhibits superior selectivity, reproducibility, low cost, as well as simple preparation and can be applied in human serum sample detection.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Life (Basel)
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
China