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Accelerating Effect of Cucurbita pepo L. Fruit Extract on Excisional Wound Healing in Depressed Rats Is Mediated through Its Anti-Inflammatory and Antioxidant Effects.
Almohaimeed, Hailah M; Al-Zahrani, Maryam Hassan; Almuhayawi, Mohammed Saad; Algaidi, Sami Awda; Batawi, Ashwaq H; Baz, Hasan Ahmed; Mohammedsaleh, Zuhair M; Baz, Nhal Ahmed; Saleh, Fayez M; Ayuob, Nasra.
Afiliação
  • Almohaimeed HM; Department of Basic Science, College of Medicine, Princess Nourah bint Abdulrahman University, Riyadh 11671, Saudi Arabia.
  • Al-Zahrani MH; Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
  • Almuhayawi MS; Department of Medical Microbiology & Parasitology, Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
  • Algaidi SA; Yousef Abdullatif Jameel Chair of Prophetic Medicine Applications (YAJCPMA), Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
  • Batawi AH; Department of Anatomy, Taibah University, Medina 42353, Saudi Arabia.
  • Baz HA; Department of Biological Science, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
  • Mohammedsaleh ZM; Clinical Toxicology, Faculty of Medicine, Umm Al Qura University/King Abdullah Medical City, Mecca 24211, Saudi Arabia.
  • Baz NA; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk 71491, Saudi Arabia.
  • Saleh FM; Department of Periodontist, King Abdullah Medical City, Mecca 24211, Saudi Arabia.
  • Ayuob N; Department of Medical Microbiology, Faculty of Medicine, University of Tabuk, Tabuk 71491, Saudi Arabia.
Nutrients ; 14(16)2022 Aug 15.
Article em En | MEDLINE | ID: mdl-36014842
ABSTRACT

Background:

Chronic stress can hinder wound healing as it suppresses both the cellular and innate immune responses.

Objectives:

The study aims to assess the effectiveness of the administration of topical and oral Cucurbita pepo L. (CP) ethanolic extract in prompting excisional wound healing in rats exposed to chronic stress, and to explain how it works. Materials and

methods:

Fifty albino rats assigned to five groups (n = 10) were utilized in this study. The chronic unpredictable mild stress (CUMS) model was used for 4 weeks to induce depressive-like behavior in rats, and a forced swim test and corticosterone were assessed to confirm its occurrence. During the experiment, an excisional wound was induced in the rats and followed. Oxidant/antioxidants status and pro-inflammatory cytokines levels were measured in the serum and wound area. Gene expression of pro-inflammatory cytokines was also assessed using RT-PCR. Wound closure histopathological changes and immunohistochemical expression of CD68, CD3, and CD4 at the wound area was assessed.

Results:

The administration of CP, both orally and topically, significantly reduced (p < 0.001) the depressive-like behavior and corticosterone and pro-inflammatory cytokines levels, while it significantly up-regulated the antioxidant activity compared to the untreated and topically CP-treated groups. Both topically CP-treated and combined CP-treated groups showed complete re-epithelialization, reduced inflammatory cells infiltration, collagen fibers deposition, and significantly increased CD3, CD4 positive T cells count, with a superior effect in the combined CP-treated groups.

Conclusion:

Cucurbita pepo L., administrated both topically and orally, can enhance the wound healing process in rats with depressive-like behavior mostly through the antioxidant, anti-inflammatory, and antidepressant activities observed in this study.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cucurbita / Antioxidantes Limite: Animals Idioma: En Revista: Nutrients Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Arábia Saudita

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cucurbita / Antioxidantes Limite: Animals Idioma: En Revista: Nutrients Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Arábia Saudita