Your browser doesn't support javascript.
loading
Genome analysis of secondary metabolite­biosynthetic gene clusters of Photorhabdus akhurstii subsp. akhurstii and its antibacterial activity against antibiotic-resistant bacteria.
Muangpat, Paramaporn; Meesil, Wipanee; Ngoenkam, Jatuporn; Teethaisong, Yothin; Thummeepak, Rapee; Sitthisak, Sutthirat; Tandhavanant, Sarunporn; Chantratita, Narisara; Bode, Helge B; Vitta, Apichat; Thanwisai, Aunchalee.
Afiliação
  • Muangpat P; Faculty of Medical Science, Department of Microbiology and Parasitology, Naresuan University, Phitsanulok, Thailand.
  • Meesil W; Faculty of Medical Science, Department of Microbiology and Parasitology, Naresuan University, Phitsanulok, Thailand.
  • Ngoenkam J; Faculty of Medical Science, Department of Microbiology and Parasitology, Naresuan University, Phitsanulok, Thailand.
  • Teethaisong Y; Faculty of Allied Health Sciences, Department of Biomedical Sciences, Burapha University, Chonburi, Thailand.
  • Thummeepak R; Research Unit for Sensor Inovation (RUSI), Burapha University, Chon Buri, Thailand.
  • Sitthisak S; Faculty of Medical Science, Department of Microbiology and Parasitology, Naresuan University, Phitsanulok, Thailand.
  • Tandhavanant S; Faculty of Medical Science, Department of Microbiology and Parasitology, Naresuan University, Phitsanulok, Thailand.
  • Chantratita N; Faculty of Tropical Medicine, Department of Microbiology and Immunology, Mahidol University, Bangkok, Thailand.
  • Bode HB; Faculty of Tropical Medicine, Department of Microbiology and Immunology, Mahidol University, Bangkok, Thailand.
  • Vitta A; Molekulare Biotechnologie, Goethe Universität Frankfurt, Frankfurt am Main, Germany.
  • Thanwisai A; Department of Natural Products in Organismic Interactions, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.
PLoS One ; 17(9): e0274956, 2022.
Article em En | MEDLINE | ID: mdl-36129957
ABSTRACT
Xenorhabdus and Photorhabdus can produce a variety of secondary metabolites with broad spectrum bioactivity against microorganisms. We investigated the antibacterial activity of Xenorhabdus and Photorhabdus against 15 antibiotic-resistant bacteria strains. Photorhabdus extracts had strong inhibitory the growth of Methicillin-resistant Staphylococcus aureus (MRSA) by disk diffusion. The P. akhurstii s subsp. akhurstii (bNN168.5_TH) extract showed lower minimum inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC). The interaction between either P. akhurstii subsp. akhurstii (bNN141.3_TH) or P. akhurstii subsp. akhurstii (bNN168.5_TH) or P. hainanensis (bNN163.3_TH) extract in combination with oxacillin determined by checkerboard assay exhibited partially synergistic interaction with fractional inhibitory concentration index (FICI) of 0.53. Time-killing assay for P. akhurstii subsp. akhurstii (bNN168.5_TH) extract against S. aureus strain PB36 significantly decreased cell viability from 105 CFU/ml to 103 CFU/ml within 30 min (P < 0.001, t-test). Transmission electron microscopic investigation elucidated that the bNN168.5_TH extract caused treated S. aureus strain PB36 (MRSA) cell membrane damage. The biosynthetic gene clusters of the bNN168.5_TH contained non-ribosomal peptide synthetase cluster (NRPS), hybrid NRPS-type l polyketide synthase (PKS) and siderophore, which identified potentially interesting bioactive products xenematide, luminmide, xenortide A-D, luminmycin A, putrebactin/avaroferrin and rhizomide A-C. This study demonstrates that bNN168.5_TH showed antibacterial activity by disrupting bacterial cytoplasmic membrane and the draft genome provided insights into the classes of bioactive products. This also provides a potential approach in developing a novel antibacterial agent.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Xenorhabdus / Photorhabdus / Staphylococcus aureus Resistente à Meticilina Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Tailândia

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Xenorhabdus / Photorhabdus / Staphylococcus aureus Resistente à Meticilina Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Tailândia