Novel nandrolone aptamer for rapid colorimetric detection of anabolic steroids.
Anal Biochem
; 658: 114937, 2022 12 01.
Article
em En
| MEDLINE
| ID: mdl-36202193
ABSTRACT
The illicit use of anabolic androgenic steroids (AAS) as performance-enhancing drugs remains a global issue threatening not only the credibility of competitive sports but also public health due to the well-documented adverse effects they elicit. AAS abuse is not restricted only to professional sports, but also extends to recreational athletes and adolescents as well as in livestock production as growth-promoting agents. Testosterone and nandrolone are among the AAS most frequently exploited. Gas chromatography-mass spectrometry is the reference method for AAS detection, but it is strictly laboratory-based and cannot be performed on-site. The great potential of aptamers in bioanalytical applications and specifically for the development of simple analytical tools suitable for on-site analysis has been extensively documented. In this report, we describe the selection and identification of aptamers binding nandrolone, exhibiting affinity dissociation constants in the low nanomolar range. A label-free colorimetric assay based on gold nanoparticles was developed using one of these novel aptamers for the detection of nandrolone and/or its metabolites. The assay could be deployed for the rapid, on-site, facile and cost-effective screening of samples and provide qualitative visual results with a red to purple/blue color change being indicative of a positive result.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dopagem Esportivo
/
Nanopartículas Metálicas
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Substâncias para Melhoria do Desempenho
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Anabolizantes
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Nandrolona
Tipo de estudo:
Diagnostic_studies
/
Qualitative_research
Limite:
Adolescent
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Humans
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Espanha