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Dispersal direction of Malaysian Fasciola gigantica from neighboring southeast Asian countries inferred using mitochondrial DNA analysis.
Ichikawa-Seki, Madoka; Hayashi, Kei; Tashiro, Michiyo; Khadijah, S.
Afiliação
  • Ichikawa-Seki M; Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan. Electronic address: madoka@iwate-u.ac.jp.
  • Hayashi K; Laboratory of Parasitology, Faculty of Veterinary Medicine, Okayama University of Science, 1-3 Ikoinooka, Imabari 794-8555, Japan.
  • Tashiro M; Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan.
  • Khadijah S; School of Food Science and Technology, University Malaysia Terengganu, 21030, Kuala Terengganu, Terengganu, Malaysia; GYST Parasitology Services, CSIRO Armidale, New England Highway, Armidale, NSW, Australia.
Infect Genet Evol ; 105: 105373, 2022 11.
Article em En | MEDLINE | ID: mdl-36202207
Fasciola gigantica and hybrid Fasciola flukes, responsible for the disease fasciolosis, are found in Southeast Asian countries. In the present study, we performed molecular species identification of Fasciola flukes distributed in Terengganu, Malaysia using multiplex PCR for phosphoenolpyruvate carboxykinase (pepck) and PCR-restriction fragment length polymorphism (RFLP) for DNA polymerase delta (pold). Simultaneously, phylogenetic analysis based on mitochondrial NADH dehydrogenase subunit 1 (nad1) was performed for the first time on Malaysian Fasciola flukes to infer the dispersal direction among neighboring countries. A total of 40 flukes used in this study were identified as F. gigantica. Eight nad1 haplotypes were identified in the F. gigantica population of Terengganu. Median-joining network analysis revealed that the Malaysian population was related to those obtained from bordering countries such as Thailand and Indonesia. However, genetic differentiation was detected using population genetics analyses. Nevertheless, the nucleotide diversity (π) value suggested that F. gigantica with the predominant haplotypes was introduced into Malaysia from Thailand and Indonesia. The dispersal direction suggested by population genetics in the present study may not be fully reliable since Fasciola flukes were collected from a single location in one state of Malaysia. Further studies analyzing more samples from many locations are required to validate the dispersal direction proposed herein.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA de Helmintos / Fasciola / Distribuição Animal Limite: Animals País/Região como assunto: Asia Idioma: En Revista: Infect Genet Evol Assunto da revista: BIOLOGIA / DOENCAS TRANSMISSIVEIS / GENETICA Ano de publicação: 2022 Tipo de documento: Article País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA de Helmintos / Fasciola / Distribuição Animal Limite: Animals País/Região como assunto: Asia Idioma: En Revista: Infect Genet Evol Assunto da revista: BIOLOGIA / DOENCAS TRANSMISSIVEIS / GENETICA Ano de publicação: 2022 Tipo de documento: Article País de publicação: Holanda