Enhanced thermal stability of the ß-galactosidase BgaB from Bacillus circulans by cyclization mediated via SpyTag/SpyCatcher interaction and its use in galacto-oligosaccharides synthesis.

Han, Mingming; Xu, Chenxi; Guan, Bo; Hu, Youzhen; Kazlauskas, Romas J; Li, Xu; Ni, Yongqing

Han, Mingming; Xu, Chenxi; Guan, Bo; Hu, Youzhen; Kazlauskas, Romas J; Li, Xu; Ni, Yongqing.

Afiliação

Han M; School of Food Science and Technology, Shihezi University, Shihezi, China.
Xu C; School of Food Science and Technology, Shihezi University, Shihezi, China.
Guan B; School of Food Science and Technology, Shihezi University, Shihezi, China. Electronic address: guanbo@shzu.edu.cn.
Hu Y; School of Food Science and Technology, Shihezi University, Shihezi, China. Electronic address: yzhu@shzu.edu.cn.
Kazlauskas RJ; Department of Biochemistry, Molecular Biology and Biophysics, The Biotechnology Institute, University of Minnesota, Saint Paul, MN, United States of America.
Li X; School of Food Science and Technology, Shihezi University, Shihezi, China.
Ni Y; School of Food Science and Technology, Shihezi University, Shihezi, China.

Int J Biol Macromol ; 222(Pt B): 2341-2352, 2022 Dec 01.

Article em En | MEDLINE | ID: mdl-36216104

Assuntos

Lactose; Oligossacarídeos; Ciclização; Lactose/metabolismo; beta-Galactosidase/química; Galactose

Palavras-chave

Aggregation; Galacto-oligosaccharides; SpyTag/SpyCatcher; Thermal stability; ß-Galactosidase

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligossacarídeos / Lactose Idioma: En Revista: Int J Biol Macromol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China País de publicação: Holanda

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