Stability of Hemoglobin Constant Spring Identified by Capillary Electrophoresis.
Lab Med
; 54(3): e91-e94, 2023 May 02.
Article
em En
| MEDLINE
| ID: mdl-36282478
ABSTRACT
OBJECTIVE:
Hemoglobin Constant Spring (HbCS) is often missed by routine hemoglobin analysis. The aim of this research was to study HbCS stability as identified by capillary electrophoresis (CE) to determine the specimen storage time limit.METHODS:
The EDTA blood of 29 HbCS samples were kept at 4°C and analyzed every workday until CE could not detect HbCS or until 7 weeks after blood collection. The genotypes were confirmed by multiplex polymerase chain reaction.RESULTS:
The median subject age was 27 years and 10 subjects were male. The HbCS levels were stable during the first 7 days but became undetectable in 5 cases (17.2%) after 1 week. All of them were heterozygous HbCS. Longer detection times were correlated with the higher baseline HbCS levels, with a correlation coefficient of 0.582 (P â ≤â 0.001).CONCLUSION:
Routine hemoglobin typing and quantitation should be performed within 1 week after blood collection to detect low HbCS levels, especially in heterozygous HbCS.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hemoglobinas Anormais
Limite:
Adult
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Lab Med
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Tailândia