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Molecular and Morphological Characterization of Eimeria crandallis Isolated from Deer (Cervidae) in Different Captive Animals.
Hafeez, Mian Abdul; Sattar, Adeel; Khalid, Kiran; Khalid, Abdur Rauf; Mahmood, Muhammad Shahid; Aleem, Muhammad Tahir; Ashraf, Kamran; Aslam, Faiza; Alouffi, Abdulaziz; Mohammed, Aymen; Almutairi, Mashal M; Ul Haq, Muhammad Ikram.
Afiliação
  • Hafeez MA; Department of Parasitology, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan.
  • Sattar A; Department of Pharmacology and Toxicology, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan.
  • Khalid K; Department of Parasitology, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan.
  • Khalid AR; Department of Livestock and Poultry Production, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan 60800, Pakistan.
  • Mahmood MS; Institute of Microbiology, University of Agriculture, Faisalabad 38000, Pakistan.
  • Aleem MT; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
  • Ashraf K; Department of Parasitology, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan.
  • Aslam F; Department of Pathology, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan.
  • Alouffi A; King Abdulaziz City for Science and Technology, Riyadh 12354, Saudi Arabia.
  • Mohammed A; Division of Molecular Therapeutics and Formulation, School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, UK.
  • Almutairi MM; Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.
  • Ul Haq MI; Veterinary Research Institute, Zarrar Shaheed Road, Lahore 54810, Pakistan.
Life (Basel) ; 12(10)2022 Oct 17.
Article em En | MEDLINE | ID: mdl-36295056
Coccidiosis is a protozoan disease that is characterized by diffuse diarrhea, dehydration, emaciation accompanied by moderate morbidity and mild mortality in animals and birds. The current study targeted the molecular characterization of Eimeria isolates in captive deer from different localities in Lahore. The host species was the Cervidae family, such as Hog deer (Axis porcinus) and Punjab urial (Ovis aries vignei). The Eimeria crandallis was isolated from zoo animals. The DNA was extracted from oocysts and amplified by using reported oligonucleotide primers that exhibited the 809 bp product. These were analyzed by using the small subunit 18S rRNA gene-based evolutionary relationship with 36 other Eimeria species reported in caprine, cervinae, bovines, avians, and rodents. Light microscopic examination exhibited 3.29% (7/213) Eimeria-positive fecal samples with morphological features, including sub-spherical forms, the presence of micropyle with polar cap, and oocysts diameters (µm) ranging from 24.32 ± 1.61 to 18.94 ± 1.51. The phylogenetic tree constitutes four distinct clusters with relatively higher values. The evolutionary network showed that sequences were clustered in the monophyletic group of Eimeria species reported in caprine and cervinae. The nucleotide and amino acid sequence similarity matrix analysis exhibited 99.5-99.9% identity of the study isolates with Eimeria crandallis (AF336339). This study provides relevant baseline data to develop strategic control measures for coccidiosis in zoo animals. However, further investigations are required to place the hog deer and Punjab urial-derived E. crandallis into the caprine-originated cluster.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Life (Basel) Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Paquistão País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Life (Basel) Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Paquistão País de publicação: Suíça