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Mechanism of PDZK1 in Hepatocellular Carcinoma Complicated with Hyperuricemia.
Guo, Linqi; Jiang, Wenda; Quan, Lingli; Teng, Xinli; Zhao, Jing; Qiu, Hongbin.
Afiliação
  • Guo L; School of Basic Medicine Jiamusi University, Jiamusi 154000, China.
  • Jiang W; Department of General Surgery, The First Affiliated Hospital of Jiamusi University, Jiamusi 154000, China.
  • Quan L; Department of General Surgery, The First Affiliated Hospital of Jiamusi University, Jiamusi 154000, China.
  • Teng X; Pulmonary and Critical Care Medicine 1, The Affiliated Zhuzhou Hospital Xiangya Medical College CSU, Zhuzhou 412000, China.
  • Zhao J; Medical Oncology, The Tumor Hospital of Jiamusi, Jiamusi 154000, China.
  • Qiu H; School of Basic Medicine Jiamusi University, Jiamusi 154000, China.
J Oncol ; 2022: 1403454, 2022.
Article em En | MEDLINE | ID: mdl-36420358
Background: Hepatocellular carcinoma (HCC) is a kind of primary liver cancer that accounts for more than 90% of primary hepatocellular carcinomas. Hyperuricemia is closely related to the development, recurrence, metastasis, and prognosis of cancer. Previous studies have proved that the serum uric acid level can increase the incidence rate and mortality of malignant tumors. However, the specific pathogenesis remains unstudied. Methods: RT-qPCR analysis showed that the mRNA expression of PDZK1 and ABCG2 increased significantly after HCC cells were exposed to different concentrations of soluble uric acid (2.5, 5, 10, 20 mg/dl) for 24 hours. Then, in HCC shRNAs, PDZK1, or over expression PDZK1 were used. CCK8, wound healing, and Transwell assay showed that PDZK1 regulates cell proliferation, invasion, and migration. Flow cytometry results revealed that PDZK1 affects cell apoptosis. Western blot results show that PDZK1 affects the STAT3/C-myc pathway. Then, in vivo tumorigenesis, allopurinol maybe an effective drug to advance: the prognosis of HCC. Results: In our study, RT-qPCR analysis showed that the mRNA expression of PDZK1 and ABCG2 increased significantly after different concentrations of soluble uric acid in HCC. Then, PDZK1 affects the proliferation, migration, and apoptosis of HCC through the STAT3/C-myc pathway. Conclusions: Hyperuricemia response affects the expression of PDZK1; PDZK1 affects the proliferation, migration, and apoptosis through the STAT3/C-myc pathway in hepatocellular carcinoma. It is suggested that PDZK1 maybe closely related to the occurrence, development, and prognosis of HCC and allopurinol maybe have potential anticancer effects.

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: J Oncol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China País de publicação: Egito

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: J Oncol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China País de publicação: Egito