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Morusin Protected Ruminal Epithelial Cells against Lipopolysaccharide-Induced Inflammation through Inhibiting EGFR-AKT/NF-κB Signaling and Improving Barrier Functions.
Yang, Chunlei; Deng, Xiangfei; Wu, Linjun; Jiang, Tianrui; Fu, Zhengwei; Li, Jinjun.
Afiliação
  • Yang C; College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China.
  • Deng X; College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China.
  • Wu L; College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China.
  • Jiang T; College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China.
  • Fu Z; College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China.
  • Li J; Institute of Food Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Int J Mol Sci ; 23(22)2022 Nov 20.
Article em En | MEDLINE | ID: mdl-36430903
ABSTRACT
Using phytogenic extracts for preventing or treating rumen epithelial inflammatory injury is a potential alternative to antibiotic use due to their residue-free characteristics. In this study, the efficacy of Morus root bark extract Morusin on ruminal epithelial cells (RECs) against pathogenic stimulus was investigated for the first time. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and quantitative real-time polymerase chain reaction (qPCR) results showed that the Morusin did not affect the cell viability of RECs and exerted anti-inflammatory effects in a concentration-dependent manner. Transcriptome analysis further revealed that the Morusin significantly downregulated the inflammatory-response-related cell signaling, while it upregulated the cell-proliferation-inhibition- and barrier-function-related processes in RECs upon lipopolysaccharide (LPS) stimulation. The epidermal growth factor receptor (EGFR) blocking and immunoblotting analysis further confirmed that the Morusin suppressed LPS-induced inflammation in RECs by downregulating the phosphorylation of protein kinase B (AKT) and nuclear factor-kappaB (NF-κB) p65 protein via inhibiting the EGFR signaling. These findings demonstrate the protective roles of Morusin in LPS-induced inflammation in RECs.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: NF-kappa B / Proteínas Proto-Oncogênicas c-akt Limite: Animals Idioma: En Revista: Int J Mol Sci Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: NF-kappa B / Proteínas Proto-Oncogênicas c-akt Limite: Animals Idioma: En Revista: Int J Mol Sci Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China