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Biofabrication of Modular Spheroids as Tumor-Scale Microenvironments for Drug Screening.
Mekhileri, Naveen Vijayan; Major, Gretel; Lim, Khoon; Mutreja, Isha; Chitcholtan, Kenny; Phillips, Elisabeth; Hooper, Gary; Woodfield, Tim.
Afiliação
  • Mekhileri NV; Department of Orthopaedic Surgery and Musculoskeletal Medicine, Centre for Bioengineering & Nanomedicine, University of Otago, Christchurch, Canterbury, 8011, New Zealand.
  • Major G; Department of Orthopaedic Surgery and Musculoskeletal Medicine, Centre for Bioengineering & Nanomedicine, University of Otago, Christchurch, Canterbury, 8011, New Zealand.
  • Lim K; Department of Orthopaedic Surgery and Musculoskeletal Medicine, Centre for Bioengineering & Nanomedicine, University of Otago, Christchurch, Canterbury, 8011, New Zealand.
  • Mutreja I; Department of Orthopaedic Surgery and Musculoskeletal Medicine, Centre for Bioengineering & Nanomedicine, University of Otago, Christchurch, Canterbury, 8011, New Zealand.
  • Chitcholtan K; Department of Obstetrics and Gynaecology, Gynaecological Cancer Research Group, University of Otago, Christchurch, Canterbury, 8011, New Zealand.
  • Phillips E; Mackenzie Cancer Research Group, Department of Pathology and Biomedical Science, University of Otago, Christchurch, Canterbury, 8011, New Zealand.
  • Hooper G; Department of Orthopaedic Surgery and Musculoskeletal Medicine, Centre for Bioengineering & Nanomedicine, University of Otago, Christchurch, Canterbury, 8011, New Zealand.
  • Woodfield T; Department of Orthopaedic Surgery and Musculoskeletal Medicine, Centre for Bioengineering & Nanomedicine, University of Otago, Christchurch, Canterbury, 8011, New Zealand.
Adv Healthc Mater ; 12(14): e2201581, 2023 06.
Article em En | MEDLINE | ID: mdl-36495232
ABSTRACT
To streamline the drug discovery pipeline, there is a pressing need for preclinical models which replicate the complexity and scale of native tumors. While there have been advancements in the formation of microscale tumor units, these models are cell-line dependent, time-consuming and have not improved clinical trial success rates. In this study, two methods for generating 3D tumor microenvironments are compared, rapidly fabricated hydrogel microspheres and traditional cell-dense spheroids. These modules are then bioassembled into 3D printed thermoplastic scaffolds, using an automated biofabrication process, to form tumor-scale models. Modules are formed with SKOV3 and HFF cells as monocultures and cocultures, and the fabrication efficiency, cell architecture, and drug response profiles are characterized, both as single modules and as multimodular constructs. Cell-encapsulated Gel-MA microspheres are fabricated with high-reproducibility and dimensions necessary for automated tumor-scale bioassembly regardless of cell type, however, only cocultured spheroids form compact modules suitable for bioassembly. Chemosensitivity assays demonstrate the reduced potency of doxorubicin in coculture bioassembled constructs and a ≈five-fold increase in drug resistance of cocultured cells in 3D modules compared with 2D monolayers. This bioassembly system is efficient and tailorable so that a variety of relevant-sized tumor constructs could be developed to study tumorigenesis and modernize drug discovery.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Esferoides Celulares / Microambiente Tumoral Tipo de estudo: Diagnostic_studies / Screening_studies Idioma: En Revista: Adv Healthc Mater Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Nova Zelândia

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Esferoides Celulares / Microambiente Tumoral Tipo de estudo: Diagnostic_studies / Screening_studies Idioma: En Revista: Adv Healthc Mater Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Nova Zelândia