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Simultaneous determination of heparan sulfate, chondroitin/dermatan sulfates, and hyaluronan glycosaminoglycan disaccharides by high-performance liquid chromatography using a reverse-phase column with adamantyl groups.
Hanamatsu, Hisatoshi; Makino, Satoshi; Ohara, Masatsugu; Suda, Goki; Yokota, Ikuko; Nishihara, Shoko; Sakamoto, Naoya; Furukawa, Jun-Ichi.
Afiliação
  • Hanamatsu H; Department of Gastroenterology and Hepatology, Graduate School of Medicine, Hokkaido University, Sapporo, Hokkaido 060-8638, Japan; Department of Orthopaedic Surgery, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Hokkaido 060-8638, Japan. Electronic address: h_ha
  • Makino S; Sanyo Fine IRICA Technology Co., Ltd. Kyoto, Kyoto 601-8037, Japan.
  • Ohara M; Department of Gastroenterology and Hepatology, Graduate School of Medicine, Hokkaido University, Sapporo, Hokkaido 060-8638, Japan.
  • Suda G; Department of Gastroenterology and Hepatology, Graduate School of Medicine, Hokkaido University, Sapporo, Hokkaido 060-8638, Japan.
  • Yokota I; Institute for Glyco-core Research (iGCORE), Nagoya University, Nagoya, Aichi 464-8601, Japan.
  • Nishihara S; Department of Bioinformatics, Graduate School of Engineering, Soka University, Hachioji, Tokyo, Japan; Glycan & Life System Integration Center (GaLSIC), Soka University, Hachioji, Tokyo, Japan.
  • Sakamoto N; Department of Gastroenterology and Hepatology, Graduate School of Medicine, Hokkaido University, Sapporo, Hokkaido 060-8638, Japan.
  • Furukawa JI; Department of Orthopaedic Surgery, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Hokkaido 060-8638, Japan; Institute for Glyco-core Research (iGCORE), Nagoya University, Nagoya, Aichi 464-8601, Japan. Electronic address: j-furu@med.nagoya-u.ac.jp.
J Chromatogr A ; 1689: 463748, 2023 Jan 25.
Article em En | MEDLINE | ID: mdl-36586283
ABSTRACT
Glycosaminoglycans (GAGs), which are one of the major components of proteoglycans, play a pivotal role in physiological processes such as signal transduction, cell adhesion, growth, and differentiation. Characterization of GAGs is challenging due to the tremendous structural diversity of heteropolysaccharides with numerous sulfate or carboxyl groups. In this present study, we examined the analysis of 2-aminobenzamide (2-AB) labeled GAG disaccharides by high-performance liquid chromatography (HPLC) using a reverse-phase (RP)-column with adamantyl groups. Under the analytical conditions, 17 types of 2-AB labeled GAG disaccharides derived from heparan sulfate, chondroitin/dermatan sulfates, and hyaluronan were sequentially separated in a single analysis. The analysis time was fast with high retention time reproducibility. Moreover, the RP-HPLC column with adamantyl groups allowed the quantification of GAGs in various biological samples, such as serum, cultured cells, and culture medium.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sulfatos de Condroitina / Glicosaminoglicanos Idioma: En Revista: J Chromatogr A Ano de publicação: 2023 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sulfatos de Condroitina / Glicosaminoglicanos Idioma: En Revista: J Chromatogr A Ano de publicação: 2023 Tipo de documento: Article