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Sclerostin Influences Exercise-Induced Adaptations in Body Composition and White Adipose Tissue Morphology in Male Mice.
Kurgan, Nigel; Stoikos, Joshua; Baranowski, Bradley J; Yumol, Jenalyn; Dhaliwal, Roopan; Sweezey-Munroe, Jake B; Fajardo, Val A; Gittings, William; Macpherson, Rebecca E K; Klentrou, Panagiota.
Afiliação
  • Kurgan N; Department of Kinesiology, Faculty of Applied Health Sciences, Brock University, St. Catharines, ON, Canada.
  • Stoikos J; Centre for Bone and Muscle Health, Brock University, St. Catharines, ON, Canada.
  • Baranowski BJ; Department of Kinesiology, Faculty of Applied Health Sciences, Brock University, St. Catharines, ON, Canada.
  • Yumol J; Centre for Bone and Muscle Health, Brock University, St. Catharines, ON, Canada.
  • Dhaliwal R; Department of Health Sciences, Faculty of Applied Health Sciences, Brock University, St. Catharines, ON, Canada.
  • Sweezey-Munroe JB; Centre for Neuroscience, Brock University, St. Catharines, ON, Canada.
  • Fajardo VA; Department of Kinesiology, Faculty of Applied Health Sciences, Brock University, St. Catharines, ON, Canada.
  • Gittings W; Centre for Bone and Muscle Health, Brock University, St. Catharines, ON, Canada.
  • Macpherson REK; Department of Health Sciences, Faculty of Applied Health Sciences, Brock University, St. Catharines, ON, Canada.
  • Klentrou P; Centre for Neuroscience, Brock University, St. Catharines, ON, Canada.
J Bone Miner Res ; 38(4): 541-555, 2023 04.
Article em En | MEDLINE | ID: mdl-36606556
Sclerostin is an inhibitor of the osteogenic Wnt/ß-catenin signaling pathway that also has an endocrine role in regulating adipocyte differentiation and metabolism. Additionally, subcutaneous white adipose tissue (scWAT) sclerostin content decreases following exercise training (EXT). Therefore, we hypothesized that EXT-induced reductions in adipose tissue sclerostin may play a role in regulating adaptations in body composition and whole-body metabolism. To test this hypothesis, 10-week-old male C57BL/6J mice were either sedentary (SED) or performing 1 hour of treadmill running at ~65% to 70% maximum oxygen consumption (VO2max ) 5 day/week (EXT) for 4 weeks and had subcutaneous injections of either saline (C) or recombinant sclerostin (S) (0.1 mg/kg body mass) 5 day/week; thus, making four groups (SED-C, EXT-C, SED-S, and EXT-S; n = 12/group). No differences in body mass were observed between experimental groups, whereas food intake was higher in EXT (p = 0.03) and S (p = 0.08) groups. There was a higher resting energy expenditure in all groups compared to SED-C. EXT-C had increased lean mass and decreased fat mass percentage compared to SED-C and SED-S. No differences in body composition were observed in either the SED-S or EXT-S groups. Lower scWAT (inguinal), epididymal white adipose tissue (eWAT) (visceral epididymal) mass, and scWAT adipocyte cell size and increased percentage of multilocular cells in scWAT were observed in the EXT-C group compared to SED-C, whereas lower eWAT was only observed in the EXT-S group. EXT mice had increased scWAT low-density lipoprotein receptor-related protein 4 (Lrp4) and mitochondrial content and sclerostin treatment only inhibited increased Lrp4 content with EXT. Together, these results provide evidence that reductions in resting sclerostin with exercise training may influence associated alterations in energy metabolism and body composition, particularly in scWAT. © 2023 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Condicionamento Físico Animal / Proteínas Adaptadoras de Transdução de Sinal Limite: Animals Idioma: En Revista: J Bone Miner Res Assunto da revista: METABOLISMO / ORTOPEDIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Canadá País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Condicionamento Físico Animal / Proteínas Adaptadoras de Transdução de Sinal Limite: Animals Idioma: En Revista: J Bone Miner Res Assunto da revista: METABOLISMO / ORTOPEDIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Canadá País de publicação: Estados Unidos