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Accuracy and processing time of kidney volume measurement methods in rodents polycystic kidney disease models: superiority of semiautomated kidney segmentation.
Doss, Mary Claire; Mullen, Sean; Roye, Ronald; Zhou, Juling; Chumley, Phillip; Mrug, Elias; Wallace, Darren P; Qian, Feng; Harris, Peter C; Yoder, Bradley K; Kim, Harrison; Mrug, Michal.
Afiliação
  • Doss MC; Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, United States.
  • Mullen S; Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, United States.
  • Roye R; Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, United States.
  • Zhou J; Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, United States.
  • Chumley P; Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, United States.
  • Mrug E; Math-Science Department, Alabama School of Fine Arts, Birmingham, Alabama, United States.
  • Wallace DP; The Jared Grantham Kidney Institute, University of Kansas Medical Center, Kansas City, Kansas, United States.
  • Qian F; Department of Medicine, University of Kansas Medical Center, Kansas City, Kansas, United States.
  • Harris PC; Division of Nephrology, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland, United States.
  • Yoder BK; Division of Nephrology and Hypertension, Department of Medicine, Mayo Clinic, Rochester, Minnesota, United States.
  • Kim H; Department of Cell, Developmental, and Integrative Biology, University of Alabama at Birmingham, Birmingham, Alabama, United States.
  • Mrug M; Department of Radiology, University of Alabama at Birmingham, Birmingham, Alabama, United States.
Am J Physiol Renal Physiol ; 324(4): F423-F430, 2023 04 01.
Article em En | MEDLINE | ID: mdl-36794756
Measurement of total kidney volume (TKV) using magnetic resonance imaging (MRI) is a valuable approach for monitoring disease progression in autosomal dominant polycystic kidney disease (PKD) and is becoming more common in preclinical studies using animal models. Manual contouring of kidney MRI areas [i.e., manual method (MM)] is a conventional, but time-consuming, way to determine TKV. We developed a template-based semiautomatic image segmentation method (SAM) and validated it in three commonly used PKD models: Cys1cpk/cpk mice, Pkd1RC/RC mice, and Pkhd1pck/pck rats (n = 10 per model). We compared SAM-based TKV with that obtained by clinical alternatives including the ellipsoid formula-based method (EM) using three kidney dimensions, the longest kidney length method (LM), and MM, which is considered the gold standard. Both SAM and EM presented high accuracy in TKV assessment in Cys1cpk/cpk mice [interclass correlation coefficient (ICC) ≥ 0.94]. SAM was superior to EM and LM in Pkd1RC/RC mice (ICC = 0.87, 0.74, and <0.10 for SAM, EM, and LM, respectively) and Pkhd1pck/pck rats (ICC = 0.59, <0.10, and <0.10, respectively). Also, SAM outperformed EM in processing time in Cys1cpk/cpk mice (3.6 ± 0.6 vs. 4.4 ± 0.7 min/kidney) and Pkd1RC/RC mice (3.1 ± 0.4 vs. 7.1 ± 2.6 min/kidney, both P < 0.001) but not in Pkhd1PCK/PCK rats (3.7 ± 0.8 vs. 3.2 ± 0.5 min/kidney). LM was the fastest (∼1 min) but correlated most poorly with MM-based TKV in all studied models. Processing times by MM were longer for Cys1cpk/cpk mice, Pkd1RC/RC mice, and Pkhd1pck.pck rats (66.1 ± 7.3, 38.3 ± 7.5, and 29.2 ± 3.5 min). In summary, SAM is a fast and accurate method to determine TKV in mouse and rat PKD models.NEW & NOTEWORTHY Total kidney volume (TKV) is a valuable readout in preclinical studies for autosomal dominant and autosomal recessive polycystic kidney diseases (ADPKD and ARPKD). Since conventional TKV assessment by manual contouring of kidney areas in all images is time-consuming, we developed a template-based semiautomatic image segmentation method (SAM) and validated it in three commonly used ADPKD and ARPKD models. SAM-based TKV measurements were fast, highly reproducible, and accurate across mouse and rat ARPKD and ADPKD models.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rim Policístico Autossômico Recessivo / Rim Policístico Autossômico Dominante Tipo de estudo: Guideline Aspecto: Patient_preference Limite: Animals Idioma: En Revista: Am J Physiol Renal Physiol Assunto da revista: FISIOLOGIA / NEFROLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rim Policístico Autossômico Recessivo / Rim Policístico Autossômico Dominante Tipo de estudo: Guideline Aspecto: Patient_preference Limite: Animals Idioma: En Revista: Am J Physiol Renal Physiol Assunto da revista: FISIOLOGIA / NEFROLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos