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Generation and comprehensive analysis of Synechococcus elongatus-Aspergillus nidulans co-culture system for polyketide production.
Feng, Jie; Li, Jingwei; Liu, Dongxia; Xin, Yuxian; Sun, Jingrong; Yin, Wen-Bing; Li, Tingting.
Afiliação
  • Feng J; School of Basic Medical Sciences, Lanzhou University, Lanzhou, 730000, China.
  • Li J; School of Basic Medical Sciences, Lanzhou University, Lanzhou, 730000, China.
  • Liu D; School of Basic Medical Sciences, Lanzhou University, Lanzhou, 730000, China.
  • Xin Y; School of Basic Medical Sciences, Lanzhou University, Lanzhou, 730000, China.
  • Sun J; School of Basic Medical Sciences, Lanzhou University, Lanzhou, 730000, China.
  • Yin WB; State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People's Republic of China.
  • Li T; School of Basic Medical Sciences, Lanzhou University, Lanzhou, 730000, China. litt@lzu.edu.cn.
Biotechnol Biofuels Bioprod ; 16(1): 32, 2023 Mar 01.
Article em En | MEDLINE | ID: mdl-36859469
BACKGROUND: Artificial microbial consortia composed of heterotrophic and photoautotrophic organisms represent a unique strategy for converting light energy and carbon dioxide into high-value bioproducts. Currently, the types of desired bioproducts are still limited, and microbial fitness benefit rendered by paired partner generally needs to be intensified. Exploring novel artificial microbial consortia at a laboratory scale is an essential step towards addressing this unmet need. This study aimed to conduct and analyze an artificial consortium composed of cyanobacterium Synechococcus elongatus FL130 with the filamentous fungus Aspergillus nidulans TWY1.1 for producing fungi-derived secondary metabolite of polyketide neosartoricin B. RESULTS: Polyketide-producing A. nidulans TWY1.1 substantially ameliorated the growth and the survival of sucrose-secreting cyanobacterium S. elongatus FL130 in salt-stressed environments. Besides sucrose, comparable amounts of other carbohydrates were released from axenically cultured FL130 cells, which could be efficiently consumed by TWY1.1. Relative to axenically cultured FL130, less glycogen was accumulated in FL130 cells co-cultured with TWY1.1, and the glycogen phosphorylase gene catalyzing the first step for glycogen degradation had two-fold expression. Different from axenically cultured filamentous fungi, abundant vacuoles were observed in fungal hyphae of TWY1.1 co-cultured with cyanobacterium FL130. Meanwhile, FL130 cells displayed a characteristic pattern of interacting with its heterotrophic partner, densely dispersing along certain hyphae of TWY1.1. Finally, polyketide neosartoricin B was produced from TWY1.1 in FL130-TWY1.1 co-cultures, which was tightly adjusted by nitrogen level. CONCLUSION: Overall, the results thoroughly proved the concept of pairing cyanobacteria with filamentous fungi to build artificial consortia for producing fungi-derived biomolecules.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Biotechnol Biofuels Bioprod Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Biotechnol Biofuels Bioprod Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China País de publicação: Reino Unido