[A method of determining inhibitory constants during reversible inhibition of enzymatic hydrolysis of a substrate]. / Metod opredeleniia ingibitornykh konstant pri obratimom tormozhenii fermentativnogo gidroliza substrata.
Ukr Biokhim Zh (1978)
; 59(5): 77-81, 1987.
Article
em Ru
| MEDLINE
| ID: mdl-3686698
ABSTRACT
Determination of inhibitory constants and antienzymic activity of reversible retardants of different type of action by the generalized inhibitory constant K sigma is estimated, results being presented. To determine more precisely the values of inhibitory constants of the competitive (Ki), noncompetitive (K'i) inhibition and of the generalized K sigma it is suggested to conduct linearization of the experimental data in coordinates 1/K sigma, s; 1/[S], where K sigma, s is a total inhibitory constant whose value depends on the substrate [S] concentration and to make calculations by the least-square method with the allowance for principal intervals of the Km values and the maximal rate of the enzymic reaction V. Comparative calculations are made by a computer using the BASIC/RT-60 language programme through the example of the acetyl choline acetyl hydrolase (EC 3.1.1.7) interaction with the quaternary phosphonium salts--reversible retardants of this enzyme.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Inibidores Enzimáticos
Limite:
Humans
Idioma:
Ru
Revista:
Ukr Biokhim Zh (1978)
Ano de publicação:
1987
Tipo de documento:
Article
País de publicação:
UA
/
UCRANIA
/
UCRÂNIA
/
UKRAINE