Nested Phosphorothioated Hybrid Primer-Mediated Isothermal Amplification for Specific and Dye-Based Subattomolar Nucleic Acid Detection at Low Temperatures.

Wang, Yaru; Zhang, Lanxiang; Shen, Yuqing; Yu, Evan Yi-Wen; Ding, Xiong

Wang, Yaru; Zhang, Lanxiang; Shen, Yuqing; Yu, Evan Yi-Wen; Ding, Xiong.

Afiliação

Wang Y; Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.
Zhang L; Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, China.
Shen Y; Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.
Yu EY; Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, China.
Ding X; Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.

ACS Sens ; 8(3): 1261-1271, 2023 03 24.

Article em En | MEDLINE | ID: mdl-36867102

Assuntos

DNA; Proteínas Proto-Oncogênicas p21(ras); Humanos; Temperatura; Proteínas Proto-Oncogênicas p21(ras)/genética; Primers do DNA/genética; Técnicas de Amplificação de Ácido Nucleico/métodos; DNA Polimerase Dirigida por DNA

Palavras-chave

KRAS oncogene; isothermal amplification; low temperatures; nucleic acid detection; phosphorothioated hybrid primers

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA / Proteínas Proto-Oncogênicas p21(ras) Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: ACS Sens Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos

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