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Extract of Bletilla formosana callus elevates cellular antioxidative activity via Nrf2/HO-1 signaling pathway and inhibits melanogenesis in zebrafish.
Wang, Ruei-Ting; Yen, Jui-Hung; Liao, Yi-Chi; Li, Yi-Zhen; Wang, Wei-Ping.
Afiliação
  • Wang RT; CHLITINA Research and Development Center, CHLITINA Holding Ltd., Taipei, 110050, Taiwan. mia.wang@tw.chlitina.com.
  • Yen JH; Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, Hsinchu, 310401, Taiwan.
  • Liao YC; Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, 402202, Taiwan.
  • Li YZ; CHLITINA Research and Development Center, CHLITINA Holding Ltd., Taipei, 110050, Taiwan.
  • Wang WP; CHLITINA Research and Development Center, CHLITINA Holding Ltd., Taipei, 110050, Taiwan.
J Genet Eng Biotechnol ; 21(1): 26, 2023 Mar 06.
Article em En | MEDLINE | ID: mdl-36877322
ABSTRACT

BACKGROUND:

Bletilla species are endangered terrestrial orchids used in natural skin care formulas in Asia for a long history. In order to explore the bioactivity potential of Bletilla species as a cosmetic ingredient in a sustainable resource manner, the callus of Bletilla formosana (Hayata) Schltr. was established and extracted by an eco-friendly supercritical fluid CO2 extraction (SFE-CO2) method. The intracellular reactive oxygen species (ROS) scavenging activity and antioxidation-related gene expression of the callus extract were evaluated in both Hs68 fibroblast cells and HaCaT keratinocytes. The melanogenesis-inhibitory effect was investigated in B16F10 melanoma cells and in an in vivo zebrafish model.

RESULTS:

The calli of B. formosana were propagated for 10-15 generations with a consistent yellow friable appearance and then subjected to SFE-CO2 extraction to obtain a yellow pasty extract. Obvious intracellular ROS scavenging activity of the extract was detected in both Hs68 and HaCaT cells with 64.30 ± 8.27% and 32.50 ± 4.05% reduction at the concentration of 250 µg/mL. Moreover, marked expression levels of heme oxygenase-1 (HO-1) and (NAD(P)H) quinone oxidoreductase-1 (NQO1) genes were detected after 6-h and 24-h treatments. These results indicate the cellular antioxidative activity of B. formosana callus extract was probably activated via the nuclear factor erythroid 2-related factor 2 (Nrf2)/HO-1 signaling pathway. Melanogenesis-inhibitory effect of the extract was observed in α-MSH stimuli-inducing B16F10 cells with 28.46% inhibition of intracellular melanin content at the concentration of 50 µg/ml. The effect was confirmed with in vivo zebrafish embryos that showed a relative pigmentation density of 80.27 ± 7.98% at the concentration of 100 µg/mL without toxicity.

CONCLUSION:

Our results shed light on a sustainable utilization of Bletilla species as a potential ingredient for skin.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: J Genet Eng Biotechnol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Taiwan

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: J Genet Eng Biotechnol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Taiwan