A robust lyophilized kit for convenient one-step formulation of [68Ga]Ga-DOTA-E-[c(RGDfK)]2 in hospital radiopharmacy for clinical PET imaging.
Appl Radiat Isot
; 196: 110725, 2023 Jun.
Article
em En
| MEDLINE
| ID: mdl-36878089
ABSTRACT
The present article describes the development of robust lyophilized kit for convenient formulation of [68Ga]Ga-DOTA-E-[c(RGDfK)]2 (E = glutamic acid, R = arginine, G = glycine, D = aspartic acid, f = phenylalanine, K = lysine) radiopharmaceutical for clinical use in non-invasive monitoring of malignancies overexpressing integrin αvß3 receptors. Five batches of the kit were prepared with optimized kit contents, all of which showed high 68Ga-radiolabeling yield (>98%). Pre-clinical evaluation of the [68Ga]Ga-radiotracer in SCID mice bearing FTC133 tumour exhibited significant accumulation in the tumor xenograft. Preliminary human clinical investigation carried out in a 60 year old male patient with metastatic lung cancer revealed high radiotracer uptake in the tumor along with satisfactory target to non-target contrast. The developed kit formulation also showed a long shelf-life of at least 12 months on storage at 0 °C. All these results point towards the promising attributes of the developed kit formulation for convenient preparation of [68Ga]Ga-DOTA-E-[c(RGDfK)]2 for routine clinical use.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tomografia por Emissão de Pósitrons
/
Radioisótopos de Gálio
Limite:
Animals
/
Humans
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Male
/
Middle aged
Idioma:
En
Revista:
Appl Radiat Isot
Assunto da revista:
MEDICINA NUCLEAR
/
SAUDE AMBIENTAL
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Índia