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Genome Editing in Chlamydomonas reinhardtii Using Cas9-gRNA Ribonucleoprotein Complex: A Step-by-Step Guide.
Dhokane, Dhananjay; Kancharla, Nagesh; Savarimuthu, Arockiasamy; Bhadra, Bhaskar; Bandyopadhyay, Anindya; Dasgupta, Santanu.
Afiliação
  • Dhokane D; Synthetic Biology Group, Reliance Corporate Park, Reliance Industries Ltd, Ghansoli, Navi Mumbai, India.
  • Kancharla N; Synthetic Biology Group, Reliance Corporate Park, Reliance Industries Ltd, Ghansoli, Navi Mumbai, India.
  • Savarimuthu A; Synthetic Biology Group, Reliance Corporate Park, Reliance Industries Ltd, Ghansoli, Navi Mumbai, India.
  • Bhadra B; Synthetic Biology Group, Reliance Corporate Park, Reliance Industries Ltd, Ghansoli, Navi Mumbai, India. bhaskar.bhadra@ril.com.
  • Bandyopadhyay A; Synthetic Biology Group, Reliance Corporate Park, Reliance Industries Ltd, Ghansoli, Navi Mumbai, India. anindya.b@ril.com.
  • Dasgupta S; Synthetic Biology Group, Reliance Corporate Park, Reliance Industries Ltd, Ghansoli, Navi Mumbai, India.
Methods Mol Biol ; 2653: 207-217, 2023.
Article em En | MEDLINE | ID: mdl-36995629
ABSTRACT
Genome editing technologies have provided opportunities to manipulate literally any genomic location, opening new avenues for reverse genetics-based improvements. Among them, CRISPR/Cas9 is the most versatile tool for genome editing applications in prokaryotes and eukaryotes. Here, we provide a guide to successfully carry out high-efficiency genome editing in Chlamydomonas reinhardtii using preassembled CRISPR/Cas9-gRNA ribonucleoprotein (RNP) complexes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Chlamydomonas reinhardtii / Edição de Genes Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Índia
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Chlamydomonas reinhardtii / Edição de Genes Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Índia