The humoral and cellular response to mRNA SARS-CoV-2 vaccine is influenced by HLA polymorphisms.
HLA
; 102(3): 301-315, 2023 09.
Article
em En
| MEDLINE
| ID: mdl-37010080
ABSTRACT
Host genetic variability contributes to susceptibility to SARS-CoV-2 infection and COVID-19 evolution and the role of HLA system has not clearly emerged, suggesting the involvement of other factors. Studying response to vaccination with Spyke protein mRNA represents an ideal model to highlight whether the humoral or cellular responses are influenced by HLA. Four hundred and sixteen workers, vaccinated with Comirnaty beginning 2021, were selected within the Azienda Ospedaliera Universitaria "Città della Salute e della Scienza di Torino." The humoral response was determined with the LIAISON® kit, while the analysis of the cellular response was performed with the Quantiferon SARS-CoV-2 assay, for the S1 (receptor-binding domain; Ag1) and S1 and S2 (Ag2) subunits of the Spyke protein. Six HLA loci were typed by next-generation sequencing. Associations between HLA and vaccine response were performed with univariate and multivariate analyses. An association was found between A*0301, B*4002 and DPB1*0601 and high antibody concentration and between A*2402, B*0801 and C*0701 and low humoral responses. The haplotype HLA-A*0101 ~ B1*0801 ~ C*0701 ~ DRB1*0301 ~ DQB1*0201 conferred an increased risk of low humoral response. Considering cellular responses, 50% of the vaccinated subjects responded against Ag1 and 59% against Ag2. Carriers of DRB1*1501 displayed a higher cellular response both to Ag1 and Ag2 compared to the rest of the cohort. Similarly, DRB1*1302 predisposed to a robust cellular response to Ag1 and Ag2, while DRB1*1104 showed an opposite trend. Cellular and humoral responses to Comirnaty are influenced by HLA. Humoral response is mainly associated to class I alleles, with A*0301, previously associated to protection against severe COVID-19, and response to vaccination, standing out. Cellular response predominantly involves class II alleles, with DRB1*1501 and DPB1*1301 prevailing. Affinity analysis for Spyke peptides is generally in line with the association results.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vacinas contra COVID-19
/
COVID-19
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
HLA
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Itália