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High-throughput screens identify a lipid nanoparticle that preferentially delivers mRNA to human tumors in vivo.
Huayamares, Sebastian G; Lokugamage, Melissa P; Rab, Regina; Da Silva Sanchez, Alejandro J; Kim, Hyejin; Radmand, Afsane; Loughrey, David; Lian, Liming; Hou, Yuning; Achyut, Bhagelu R; Ehrhardt, Annette; Hong, Jeong S; Sago, Cory D; Paunovska, Kalina; Echeverri, Elisa Schrader; Vanover, Daryll; Santangelo, Philip J; Sorscher, Eric J; Dahlman, James E.
Afiliação
  • Huayamares SG; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
  • Lokugamage MP; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
  • Rab R; Winship Cancer Institute, Emory University, Atlanta, GA 30322, USA.
  • Da Silva Sanchez AJ; Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA 30332, USA; Department of Chemical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
  • Kim H; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
  • Radmand A; Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, GA 30332, USA; Department of Chemical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
  • Loughrey D; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
  • Lian L; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
  • Hou Y; Winship Cancer Institute, Emory University, Atlanta, GA 30322, USA.
  • Achyut BR; Department of Pediatrics, Emory University, Atlanta, GA 30322, USA.
  • Ehrhardt A; Department of Pediatrics, Emory University, Atlanta, GA 30322, USA.
  • Hong JS; Department of Pediatrics, Emory University, Atlanta, GA 30322, USA.
  • Sago CD; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
  • Paunovska K; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
  • Echeverri ES; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
  • Vanover D; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
  • Santangelo PJ; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
  • Sorscher EJ; Department of Pediatrics, Emory University, Atlanta, GA 30322, USA; Winship Cancer Institute, Emory University, Atlanta, GA 30322, USA. Electronic address: esorscher@emory.edu.
  • Dahlman JE; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA. Electronic address: james.dahlman@bme.gatech.edu.
J Control Release ; 357: 394-403, 2023 05.
Article em En | MEDLINE | ID: mdl-37028451
ABSTRACT
Lipid nanoparticles (LNPs) are a clinically relevant way to deliver therapeutic mRNA to hepatocytes in patients. However, LNP-mRNA delivery to end-stage solid tumors such as head and neck squamous cell carcinoma (HNSCC) remains more challenging. While scientists have used in vitro assays to evaluate potential nanoparticles for HNSCC delivery, high-throughput delivery assays performed directly in vivo have not been reported. Here we use a high-throughput LNP assay to evaluate how 94 chemically distinct nanoparticles delivered nucleic acids to HNSCC solid tumors in vivo. DNA barcodes were used to identify LNPHNSCC, a novel LNP for systemic delivery to HNSCC solid tumors. Importantly, LNPHNSCC retains tropism to HNSCC solid tumors while minimizing off-target delivery to the liver.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nanopartículas / Neoplasias de Cabeça e Pescoço Limite: Humans Idioma: En Revista: J Control Release Assunto da revista: FARMACOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nanopartículas / Neoplasias de Cabeça e Pescoço Limite: Humans Idioma: En Revista: J Control Release Assunto da revista: FARMACOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos