Development of a Golgi-targeted superoxide anion fluorescent probe for elucidating protein GOLPH3 function in myocardial ischemia-reperfusion injury.
Anal Chim Acta
; 1255: 341100, 2023 May 15.
Article
em En
| MEDLINE
| ID: mdl-37032049
ABSTRACT
Superoxide anion (O2â¢-) is an important reactive oxygen species (ROS) and participates in various physiological and pathological processes in the organism. The O2â¢- burst induced by ischemia-reperfusion (I/R) is associated with cardiovascular disease and promotes the cell apoptosis. In this work, a turn-on type Golgi-targeting fluorescent probe Gol-Cou-O2â¢- was rationally designed for sensitive and selective detection of O2â¢-. The minimum detection limit concentration for O2â¢- was about 3.9 × 10-7 M in aqueous solution. Gol-Cou-O2â¢- showed excellent capacity of detecting exogenous and endogenous O2â¢- in living cells and zebrafish, and was also used to capture the up-regulated O2â¢- level during the duration of I/R process in cardiomyocytes. Golgi Phosphoprotein 3 (GOLPH3) is a potential Golgi stress marker protein and plays a key role in cells apoptosis during I/R. The fluorescence imaging and flow cytometry assay results indicated that silencing GOLPH3 through siRNA could give rise to the down-regulated O2â¢- level and alleviation of apoptosis in I/R myocardial cells. Thus, development of Gol-Cou-O2â¢- provides a diagnostic tool for myocardial oxidative stress injury and distinct insights on roles of GOLPH3 in myocardial I/R injury.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Traumatismo por Reperfusão Miocárdica
/
Superóxidos
Limite:
Animals
Idioma:
En
Revista:
Anal Chim Acta
Ano de publicação:
2023
Tipo de documento:
Article