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Borrelia burgdorferi Engages Mammalian Type I IFN Responses via the cGAS-STING Pathway.
Farris, Lauren C; Torres-Odio, Sylvia; Adams, L Garry; West, A Phillip; Hyde, Jenny A.
Afiliação
  • Farris LC; Department of Microbial Pathogenesis and Immunology, School of Medicine, Texas A&M University, Bryan, TX.
  • Torres-Odio S; Department of Microbial Pathogenesis and Immunology, School of Medicine, Texas A&M University, Bryan, TX.
  • Adams LG; Department of Veterinary Pathobiology, School of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX.
  • West AP; Department of Microbial Pathogenesis and Immunology, School of Medicine, Texas A&M University, Bryan, TX.
  • Hyde JA; Department of Microbial Pathogenesis and Immunology, School of Medicine, Texas A&M University, Bryan, TX.
J Immunol ; 210(11): 1761-1770, 2023 06 01.
Article em En | MEDLINE | ID: mdl-37067290
Borrelia burgdorferi, the etiologic agent of Lyme disease, is a spirochete that modulates numerous host pathways to cause a chronic, multisystem inflammatory disease in humans. B. burgdorferi infection can lead to Lyme carditis, neurologic complications, and arthritis because of the ability of specific borrelial strains to disseminate, invade, and drive inflammation. B. burgdorferi elicits type I IFN (IFN-I) responses in mammalian cells and tissues that are associated with the development of severe arthritis or other Lyme-related complications. However, the innate immune sensors and signaling pathways controlling IFN-I induction remain unclear. In this study, we examined whether intracellular nucleic acid sensing is required for the induction of IFN-I to B. burgdorferi. Using fluorescence microscopy, we show that B. burgdorferi associates with mouse and human cells in culture, and we document that internalized spirochetes colocalize with the pattern recognition receptor cyclic GMP-AMP synthase (cGAS). Moreover, we report that IFN-I responses in mouse macrophages and murine embryonic fibroblasts are significantly attenuated in the absence of cGAS or its adaptor stimulator of IFN genes (STING), which function to sense and respond to intracellular DNA. Longitudinal in vivo tracking of bioluminescent B. burgdorferi revealed similar dissemination kinetics and borrelial load in C57BL/6J wild-type, cGAS-deficient, or STING-deficient mice. However, infection-associated tibiotarsal joint pathology and inflammation were modestly reduced in cGAS-deficient compared with wild-type mice. Collectively, these results indicate that the cGAS-STING pathway is a critical mediator of mammalian IFN-I signaling and innate immune responses to B. burgdorferi.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Artrite / Doença de Lyme / Interferon Tipo I / Borrelia burgdorferi Limite: Animals / Humans Idioma: En Revista: J Immunol Ano de publicação: 2023 Tipo de documento: Article País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Artrite / Doença de Lyme / Interferon Tipo I / Borrelia burgdorferi Limite: Animals / Humans Idioma: En Revista: J Immunol Ano de publicação: 2023 Tipo de documento: Article País de publicação: Estados Unidos