Itraconazole Modulates Phospholipid Levels in Tumor-associated Macrophages.
Anticancer Res
; 43(5): 1981-1984, 2023 May.
Article
em En
| MEDLINE
| ID: mdl-37097657
ABSTRACT
BACKGROUND/AIM:
Itraconazole, an antifungal drug, repolarizes pro-tumorigenic M2 tumor-associated macrophages to anti-tumorigenic M1-like phenotypes, thereby inhibiting the proliferation of cancer cells; however, the underlying mechanism remains unclear. Therefore, we investigated the effect of itraconazole on membrane-associated lipids in tumor-associated macrophages (TAM). MATERIALS ANDMETHODS:
M1 and M2 macrophages were derived from the human monocyte leukemia cell line (THP-1) and cultured with or without 10 µM itraconazole. Cells were homogenized and subjected to liquid chromatography/mass spectrometry (LC/MS) analysis to estimate the glycerophospholipid levels in the cells.RESULTS:
Lipidomic analysis results, displayed on a volcano plot, revealed that itraconazole-induced altered phospholipid composition, with more pronounced changes in M2 macrophages than in M1. Notably, itraconazole significantly increased intracellular phosphatidylinositol and lysophosphatidylcholine levels in M2 macrophages.CONCLUSION:
Itraconazole modulates the lipid metabolism of TAMs, which could have implications for the development of novel cancer therapies.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Itraconazol
/
Macrófagos Associados a Tumor
Tipo de estudo:
Risk_factors_studies
Limite:
Humans
Idioma:
En
Revista:
Anticancer Res
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Japão