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Single-cell RNA sequencing reveals peripheral blood mononuclear immune cell landscape associated with operational tolerance in a kidney transplant recipient.
Azim, Shafquat; Zubair, Haseeb; Rousselle, Thomas; McDaniels, Jennifer M; Shetty, Amol C; Kuscu, Cem; Kuscu, Canan; Talwar, Manish; Eason, James D; Maluf, Daniel G; Mas, Valeria R.
Afiliação
  • Azim S; Surgical Sciences Division, Department of Surgery, University of Maryland School of Medicine, Baltimore, Maryland, USA.
  • Zubair H; Surgical Sciences Division, Department of Surgery, University of Maryland School of Medicine, Baltimore, Maryland, USA.
  • Rousselle T; Surgical Sciences Division, Department of Surgery, University of Maryland School of Medicine, Baltimore, Maryland, USA.
  • McDaniels JM; Surgical Sciences Division, Department of Surgery, University of Maryland School of Medicine, Baltimore, Maryland, USA.
  • Shetty AC; Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, Maryland, USA.
  • Kuscu C; Department of Surgery, Transplant Research Institute, College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee University of Tennessee Health Science Center, Memphis, Tennessee, USA.
  • Kuscu C; Department of Surgery, Transplant Research Institute, College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee University of Tennessee Health Science Center, Memphis, Tennessee, USA.
  • Talwar M; James D. Eason Transplant Institute, University of Tennessee Health Science Center, Memphis, Tennessee, USA.
  • Eason JD; James D. Eason Transplant Institute, University of Tennessee Health Science Center, Memphis, Tennessee, USA.
  • Maluf DG; Program in Transplantation, University of Maryland School of Medicine, Baltimore, Maryland, USA.
  • Mas VR; Surgical Sciences Division, Department of Surgery, University of Maryland School of Medicine, Baltimore, Maryland, USA. Electronic address: vmas@som.umaryland.edu.
Am J Transplant ; 23(9): 1434-1445, 2023 09.
Article em En | MEDLINE | ID: mdl-37201755
Operational tolerance (OT) after kidney transplantation is defined as stable graft acceptance without the need for immunosuppression therapy. However, it is not clear which cellular and molecular pathways are driving tolerance in these patients. In this first-of-its-kind pilot study, we assessed the immune landscape associated with OT using single-cell analyses. Peripheral mononuclear cells from a kidney transplant recipient with OT (Tol), 2 healthy individuals (HC), and a kidney transplant recipient with normal kidney function on standard-of-care immunosuppression (SOC) were evaluated. The immune landscape of the Tol was drastically different from that of SOC and emerged closer to the profile of HC. TCL1A+ naive B cells and LSGAL1+ regulatory T cells (Tregs) were in higher proportions in Tol. We were unable to identify the Treg subcluster in SOC. The ligand-receptor analysis in HC and Tol identified interactions between B cells, and Tregs that enhance the proliferation and suppressive function of Tregs. SOC reported the highest proportion of activated B cells with more cells in the G2M phase. Our single-cell RNA sequencing study identified the mediators of tolerance; however, it emphasizes the requirement of similar investigations on a larger cohort to reaffirm the role of immune cells in tolerance.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transplante de Rim Tipo de estudo: Etiology_studies / Risk_factors_studies Limite: Humans Idioma: En Revista: Am J Transplant Assunto da revista: TRANSPLANTE Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transplante de Rim Tipo de estudo: Etiology_studies / Risk_factors_studies Limite: Humans Idioma: En Revista: Am J Transplant Assunto da revista: TRANSPLANTE Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Estados Unidos