Protocol for biallelic tagging of an endogenous gene using CRISPR-Cas9 in human cells.
STAR Protoc
; 4(2): 102286, 2023 May 29.
Article
em En
| MEDLINE
| ID: mdl-37252842
ABSTRACT
We present here a protocol for biallelic tagging of an endogenous gene in human cells using CRISPR-Cas9 editing technology. Using RIF1 as an example, we describe tagging the gene with a mini-auxin-inducible degron and a green fluorescent protein at its C terminus. We detail steps for preparing and designing the sgRNA and homologous repair template, and clone selection and verification. For complete details on the use and execution of this protocol, please refer to Kong et al.1.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2023
Tipo de documento:
Article