Detection of inherited adenylosuccinase deficiency by two dimensional thin layer chromatography of urinary imidazoles.
Adv Exp Med Biol
; 195 Pt A: 21-5, 1986.
Article
em En
| MEDLINE
| ID: mdl-3728154
ABSTRACT
Patients with inherited adenylosuccinase deficiency excrete large quantities of succinyloaminoimidazolecarboxamide riboside (SAICAR) and succinyloadenosine (SAdo). A two dimensional thin layer chromatography method for the detection of SAICAR is described. The method consists of 1 isolation of imidazoles with a cation exchange resin; 2 tlc on cellulose plates, solvent I isopropanol-ammonia 10% (41) and II butanol-acetic acid-water (411); detection with Pauly reagent. SAICAR gives rise to an isolated spot with a characteristic bluish color. Also a simple one dimensional thin layer chromatography method for screening of high risk populations is given. Four new cases could be diagnosed. Clinical and chemical data, including concentrations of SAICAR and SAdo in urine, plasma and cerebrospinal fluid, determined by column chromatography, are presented.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Erros Inatos do Metabolismo da Purina-Pirimidina
/
Adenosina
/
Adenilossuccinato Liase
/
Liases
Tipo de estudo:
Diagnostic_studies
Limite:
Child
/
Female
/
Humans
/
Infant
/
Male
/
Newborn
Idioma:
En
Revista:
Adv Exp Med Biol
Ano de publicação:
1986
Tipo de documento:
Article