Isolation of Extracellular Vesicles by a Microfluidic Platform to Diagnose and Monitor Pancreatic Cancer.
Methods Mol Biol
; 2679: 181-191, 2023.
Article
em En
| MEDLINE
| ID: mdl-37300616
ABSTRACT
Exosomes are extracellular vesicles that are involved in cell-cell communication. Considering their bioavailability and accessibility in all the body fluids (including the blood, semen, breast milk, saliva, and urine), their use has been proposed as an alternative noninvasive tool for the diagnosis, monitoring, and prognosis of several diseases, including cancer. The isolation of exosomes and their subsequent analysis are emerging as a promising technique in diagnostics and personalized medicine. The most widely employed isolation procedure is differential ultracentrifugation, but this approach is laborious, time-consuming, and expensive and with limited isolation yield. Microfluidic devices are now emerging as novel platforms for exosome isolation, which is a low cost technology and enables high purity and fast treatment of exosome isolation. Our approach describes a microfluidic device that enables inflow capture and separation from whole blood using antibody-functionalized magnetic nanoparticles. This device allows isolation of pancreatic cancer-derived exosomes from whole blood without the need of any pretreatment, resulting in a high sensitivity.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Pancreáticas
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Técnicas Analíticas Microfluídicas
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Exossomos
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Vesículas Extracelulares
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Espanha