A Simple, Reproducible Procedure for Chemiluminescent Western Blot Quantification.
Bio Protoc
; 13(9): e4667, 2023 May 05.
Article
em En
| MEDLINE
| ID: mdl-37323629
ABSTRACT
Western blotting is a universally used technique to identify specific proteins from a heterogeneous and complex mixture. However, there is no clear and common procedure to quantify the results obtained, resulting in variations due to the different software and protocols used in each laboratory. Here, we have developed a procedure based on the increase in chemiluminescent signal to obtain a representative value for each band to be quantified. Images were processed with ImageJ and subsequently compared using R software. The result is a linear regression model in which we use the slope of the signal increase within the combined linear range of detection to compare between samples. This approach allows to quantify and compare protein levels from different conditions in a simple and reproducible way. Graphical overview.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Bio Protoc
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Espanha