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A positively tuned voltage indicator for extended electrical recordings in the brain.
Evans, S Wenceslao; Shi, Dong-Qing; Chavarha, Mariya; Plitt, Mark H; Taxidis, Jiannis; Madruga, Blake; Fan, Jiang Lan; Hwang, Fuu-Jiun; van Keulen, Siri C; Suomivuori, Carl-Mikael; Pang, Michelle M; Su, Sharon; Lee, Sungmoo; Hao, Yukun A; Zhang, Guofeng; Jiang, Dongyun; Pradhan, Lagnajeet; Roth, Richard H; Liu, Yu; Dorian, Conor C; Reese, Austin L; Negrean, Adrian; Losonczy, Attila; Makinson, Christopher D; Wang, Sui; Clandinin, Thomas R; Dror, Ron O; Ding, Jun B; Ji, Na; Golshani, Peyman; Giocomo, Lisa M; Bi, Guo-Qiang; Lin, Michael Z.
Afiliação
  • Evans SW; Department of Neurobiology, Stanford University Medical Center, Stanford, CA, USA.
  • Shi DQ; Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.
  • Chavarha M; Department of Bioengineering, Stanford University, Stanford, CA, USA.
  • Plitt MH; Department of Neurobiology, Stanford University Medical Center, Stanford, CA, USA.
  • Taxidis J; Department of Neurology, UCLA David Geffen School of Medicine, Los Angeles, CA, USA.
  • Madruga B; Program in Neurosciences & Mental Health, Hospital for Sick Children, Toronto, Ontario, Canada.
  • Fan JL; Department of Physiology, University of Toronto, Toronto, Ontario, Canada.
  • Hwang FJ; Program in Neurosciences & Mental Health, Hospital for Sick Children, Toronto, Ontario, Canada.
  • van Keulen SC; Department of Physiology, University of Toronto, Toronto, Ontario, Canada.
  • Suomivuori CM; Department of Neurology, UCLA David Geffen School of Medicine, Los Angeles, CA, USA.
  • Pang MM; UC Berkeley/UCSF Joint Program in Bioengineering, University of California Berkeley, Berkeley, CA, USA.
  • Su S; Department of Neurosurgery, Stanford University Medical Center, Stanford, CA, USA.
  • Lee S; Department of Computer Science, Stanford University, Stanford, CA, USA.
  • Hao YA; Department of Computer Science, Stanford University, Stanford, CA, USA.
  • Zhang G; Department of Neurobiology, Stanford University Medical Center, Stanford, CA, USA.
  • Jiang D; Department of Neurobiology, Stanford University Medical Center, Stanford, CA, USA.
  • Pradhan L; Department of Neurobiology, Stanford University Medical Center, Stanford, CA, USA.
  • Roth RH; Department of Neurobiology, Stanford University Medical Center, Stanford, CA, USA.
  • Liu Y; Department of Bioengineering, Stanford University, Stanford, CA, USA.
  • Dorian CC; Department of Neurobiology, Stanford University Medical Center, Stanford, CA, USA.
  • Reese AL; Department of Neurobiology, Stanford University Medical Center, Stanford, CA, USA.
  • Negrean A; Department of Neurobiology, Stanford University Medical Center, Stanford, CA, USA.
  • Losonczy A; Department of Neurosurgery, Stanford University Medical Center, Stanford, CA, USA.
  • Makinson CD; Department of Neurosurgery, Stanford University Medical Center, Stanford, CA, USA.
  • Wang S; Department of Ophthalmology, Stanford University Medical Center, Stanford, CA, USA.
  • Clandinin TR; Department of Neurology, UCLA David Geffen School of Medicine, Los Angeles, CA, USA.
  • Dror RO; Institute for Genomic Medicine, Columbia University, New York, NY, USA.
  • Ding JB; Department of Neuroscience, Columbia University, New York, NY, USA.
  • Ji N; Department of Neuroscience, Columbia University, New York, NY, USA.
  • Golshani P; Mortimer B. Zuckerman Mind Brain Behavior Institute, New York, NY, USA.
  • Giocomo LM; Kavli Institute for Brain Science, New York, NY, USA.
  • Bi GQ; Institute for Genomic Medicine, Columbia University, New York, NY, USA.
  • Lin MZ; Department of Neurology, Columbia University, New York, NY, USA.
Nat Methods ; 20(7): 1104-1113, 2023 07.
Article em En | MEDLINE | ID: mdl-37429962
ABSTRACT
Genetically encoded voltage indicators (GEVIs) enable optical recording of electrical signals in the brain, providing subthreshold sensitivity and temporal resolution not possible with calcium indicators. However, one- and two-photon voltage imaging over prolonged periods with the same GEVI has not yet been demonstrated. Here, we report engineering of ASAP family GEVIs to enhance photostability by inversion of the fluorescence-voltage relationship. Two of the resulting GEVIs, ASAP4b and ASAP4e, respond to 100-mV depolarizations with ≥180% fluorescence increases, compared with the 50% fluorescence decrease of the parental ASAP3. With standard microscopy equipment, ASAP4e enables single-trial detection of spikes in mice over the course of minutes. Unlike GEVIs previously used for one-photon voltage recordings, ASAP4b and ASAP4e also perform well under two-photon illumination. By imaging voltage and calcium simultaneously, we show that ASAP4b and ASAP4e can identify place cells and detect voltage spikes with better temporal resolution than commonly used calcium indicators. Thus, ASAP4b and ASAP4e extend the capabilities of voltage imaging to standard one- and two-photon microscopes while improving the duration of voltage recordings.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Encéfalo / Cálcio Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Encéfalo / Cálcio Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos