Yeast Two-Hybrid Technique to Identify Protein-Protein Interactions.
Methods Mol Biol
; 2690: 1-8, 2023.
Article
em En
| MEDLINE
| ID: mdl-37450132
ABSTRACT
Protein-protein interactions are specific and direct physical contact between two or more proteins, and the interaction involves hydrogen bonding, electrostatic forces, and hydrophobic forces. Majority of biological processes in the living cell are executed by proteins, and any particular protein function is regulated by numerous other proteins. Thus, knowledge of protein-protein interaction is necessary to understand the biological processes. In this chapter, we explain the widely used yeast two-hybrid assay to identify the protein-interacting partners.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Proteínas
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Estados Unidos