Forster Resonance Energy Transfer (FRET) to Visualize Protein-Protein Interactions in the Plant Cell.

ABSTRACT

Forster resonance energy transfer (FRET) is an efficient method to visualize the protein-protein interaction in living cells. This technique is based on transfer of energy between two different fluorophores that are fused to two interacting proteins. In this chapter, we described the FRET assay to visualize the protein-protein interaction in plant cells.