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Isolation, Characterization, and Comparative Genomics of the Novel Potential Probiotics from Canine Feces.
Foongsawat, Ngamlak; Sunthornthummas, Sirinthorn; Nantavisai, Kwannan; Surachat, Komwit; Rangsiruji, Achariya; Sarawaneeyaruk, Siriruk; Insian, Kedvadee; Sukontasing, Sirapan; Suwannasai, Nuttika; Pringsulaka, Onanong.
Afiliação
  • Foongsawat N; Department of Microbiology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand.
  • Sunthornthummas S; National Biobank of Thailand (NBT), National Science and Technology Development Agency, Pathum Thani 12120, Thailand.
  • Nantavisai K; Department of Microbiology, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, Thailand.
  • Surachat K; Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla 90110, Thailand.
  • Rangsiruji A; Translational Medicine Research Center, Faculty of Medicine, Prince of Songkla University, Songkhla 90110, Thailand.
  • Sarawaneeyaruk S; Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand.
  • Insian K; Department of Microbiology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand.
  • Sukontasing S; Department of Microbiology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand.
  • Suwannasai N; Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand.
  • Pringsulaka O; Department of Microbiology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand.
Food Sci Anim Resour ; 43(4): 685-702, 2023 Jul.
Article em En | MEDLINE | ID: mdl-37484003
ABSTRACT
Lactic acid bacteria (LAB) are commonly used as probiotics; however, not all LAB strains have the same beneficial effects. To successfully use LAB as probiotics in canines, LAB species should originate from the canine intestinal tract as they display host specificity. The objective of this study was to investigate the phenotypic and genomic traits of potential probiotic LAB isolated from canine fecal samples. Twenty LAB samples were evaluated for their potential probiotic characteristics including resistance to low pH, bile salts, hydrophobicity, auto-aggregation, co-aggregation, adhesion to epithelia or mucosa, and production of inhibitory compounds. Additionally, we evaluated their safety and other beneficial effects on canine health, such as DPPH free radical scavenging, and ß-galactosidase. Four strains demonstrated potential probiotic characteristics and were selected Enterococcus hirae Pom4, Limosilactobacillus fermentum Pom5, Pediococcus pentosaceus Chi8, and Ligilactobacillus animalis FB2. Safety evaluations showed that all strains lacked hemolytic activity, could not produce biogenic amines, and did not carry any pathogenic genes. In addition, L. fermentum Pom5 and P. pentosaceus Chi8 displayed susceptibility to all antibiotics and concordant with the absence of antibiotic resistance genes. Based on their phenotypic and genomic characteristics, L. fermentum Pom5 and P. pentosaceus Chi8 were identified as potential probiotic candidates for canines.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: Food Sci Anim Resour Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Tailândia

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: Food Sci Anim Resour Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Tailândia