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Multiple Clonostachys rosea UDP-Glycosyltransferases Contribute to the Production of 15-Acetyl-Deoxynivalenol-3-O-Glycoside When Confronted with Fusarium graminearum.
Robinson, Kelly A; St-Jacques, Antony D; Shields, Sam W; Sproule, Amanda; Demissie, Zerihun A; Overy, David P; Loewen, Michele C.
Afiliação
  • Robinson KA; Aquatic and Crop Resources Development Research Center, National Research Council of Canada, 100 Sussex Drive, Ottawa, ON K1A 0R6, Canada.
  • St-Jacques AD; Aquatic and Crop Resources Development Research Center, National Research Council of Canada, 100 Sussex Drive, Ottawa, ON K1A 0R6, Canada.
  • Shields SW; Ottawa Research and Development Center, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0Z2, Canada.
  • Sproule A; Ottawa Research and Development Center, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0Z2, Canada.
  • Demissie ZA; Aquatic and Crop Resources Development Research Center, National Research Council of Canada, 100 Sussex Drive, Ottawa, ON K1A 0R6, Canada.
  • Overy DP; Ottawa Research and Development Center, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0Z2, Canada.
  • Loewen MC; Aquatic and Crop Resources Development Research Center, National Research Council of Canada, 100 Sussex Drive, Ottawa, ON K1A 0R6, Canada.
J Fungi (Basel) ; 9(7)2023 Jul 02.
Article em En | MEDLINE | ID: mdl-37504712
Mycotoxins, derived from toxigenic fungi such as Fusarium, Aspergillus, and Penicillium species have impacted the human food chain for thousands of years. Deoxynivalenol (DON), is a tetracyclic sesquiterpenoid type B trichothecene mycotoxin predominantly produced by F. culmorum and F. graminearum during the infection of corn, wheat, oats, barley, and rice. Glycosylation of DON is a protective detoxification mechanism employed by plants. More recently, DON glycosylating activity has also been detected in fungal microparasitic (biocontrol) fungal organisms. Here we follow up on the reported conversion of 15-acetyl-DON (15-ADON) into 15-ADON-3-O-glycoside (15-ADON-3G) in Clonostachys rosea. Based on the hypothesis that the reaction is likely being carried out by a uridine diphosphate glycosyl transferase (UDP-GTase), we applied a protein structural comparison strategy, leveraging the availability of the crystal structure of rice Os70 to identify a subset of potential C. rosea UDP-GTases that might have activity against 15-ADON. Using CRISPR/Cas9 technology, we knocked out several of the selected UDP-GTases in the C. rosea strain ACM941. Evaluation of the impact of knockouts on the production of 15-ADON-3G in confrontation assays with F. graminearum revealed multiple UDP-GTase enzymes, each contributing partial activities. The relationship between these positive hits and other UDP-GTases in fungal and plant species is discussed.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: J Fungi (Basel) Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Canadá País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: J Fungi (Basel) Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Canadá País de publicação: Suíça