Feasibility and safety of synchrotron-based X-ray phase contrast imaging as a technique complementary to histopathology analysis.
Histochem Cell Biol
; 160(5): 377-389, 2023 Nov.
Article
em En
| MEDLINE
| ID: mdl-37523091
ABSTRACT
X-ray phase contrast imaging (X-PCI) is a powerful technique for high-resolution, three-dimensional imaging of soft tissue samples in a non-destructive manner. In this technical report, we assess the quality of standard histopathological techniques performed on formalin-fixed, paraffin-embedded (FFPE) human tissue samples that have been irradiated with different doses of X-rays in the context of an X-PCI experiment. The data from this study demonstrate that routine histochemical and immunohistochemical staining quality as well as DNA and RNA analyses are not affected by previous X-PCI on human FFPE samples. From these data we conclude it is feasible and acceptable to perform X-PCI on FFPE human biopsies.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Síncrotrons
/
Intervenção Coronária Percutânea
Limite:
Humans
Idioma:
En
Revista:
Histochem Cell Biol
Assunto da revista:
CITOLOGIA
/
HISTOCITOQUIMICA
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Reino Unido