Massively Parallel Sequencing of the Mitogenome from Human Hair Shafts in Forensic Investigations.
Curr Protoc
; 3(8): e865, 2023 Aug.
Article
em En
| MEDLINE
| ID: mdl-37579069
ABSTRACT
This article highlights methods used to perform DNA extraction, mitochondrial DNA quantification, multiplex PCR amplification, amplicon-based massively parallel sequencing, and data analysis of the mitochondrial genome (mitogenome) from human hair shafts. The focus is on applications to forensic casework, but this set of protocols can be used for any purpose involving small cuttings (as small as 1 to 5 mm) of human hair shafts up to 40 years from the time of collection. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1 Extraction of mitochondrial DNA from human hair shafts Basic Protocol 2 Quantification of mitochondrial DNA (copies/µl) Basic Protocol 3 Multiplex amplification of the mitogenome Basic Protocol 4 Library preparation and sequencing of mitogenome amplicons Basic Protocol 5 Data analysis of mitogenome haplotypes.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Genoma Mitocondrial
Limite:
Humans
Idioma:
En
Revista:
Curr Protoc
Ano de publicação:
2023
Tipo de documento:
Article