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Optimizing cryopreservation of nasal polyp tissue for cellular functional studies and single-cell RNA sequencing.
Sohail, Aaqib; Baloh, Carolyn H; Hacker, Jonathan; Cho, Laura; Ryan, Tessa; Bergmark, Regan W; Lee, Stella E; Maxfield, Alice; Roditi, Rachel; Dwyer, Daniel F; Buchheit, Kathleen M; Laidlaw, Tanya M.
Afiliação
  • Sohail A; Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA.
  • Baloh CH; Division of Allergy and Clinical Immunology, Brigham and Women's Hospital, Boston, Massachusetts, USA.
  • Hacker J; Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA.
  • Cho L; Division of Allergy and Clinical Immunology, Brigham and Women's Hospital, Boston, Massachusetts, USA.
  • Ryan T; Division of Allergy and Clinical Immunology, Brigham and Women's Hospital, Boston, Massachusetts, USA.
  • Bergmark RW; Division of Allergy and Clinical Immunology, Brigham and Women's Hospital, Boston, Massachusetts, USA.
  • Lee SE; Division of Allergy and Clinical Immunology, Brigham and Women's Hospital, Boston, Massachusetts, USA.
  • Maxfield A; Division of Otolaryngology-Head & Neck Surgery, Department of Surgery, Harvard Medical School, Brigham and Women's Hospital, Boston, Massachusetts, USA.
  • Roditi R; Division of Otolaryngology-Head & Neck Surgery, Department of Surgery, Harvard Medical School, Brigham and Women's Hospital, Boston, Massachusetts, USA.
  • Dwyer DF; Division of Otolaryngology-Head & Neck Surgery, Department of Surgery, Harvard Medical School, Brigham and Women's Hospital, Boston, Massachusetts, USA.
  • Buchheit KM; Division of Otolaryngology-Head & Neck Surgery, Department of Surgery, Harvard Medical School, Brigham and Women's Hospital, Boston, Massachusetts, USA.
  • Laidlaw TM; Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA.
Int Forum Allergy Rhinol ; 2023 Sep 23.
Article em En | MEDLINE | ID: mdl-37742089
ABSTRACT
KEY POINTS Mast cell numbers were reduced in samples cryopreserved as whole tissue chunks. Thawed epithelial cells had reduced proliferation rates when preserved as dissociated cell suspensions. The right cryopreservation method to choose may depend on the goals and cell-type focus of the project.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Int Forum Allergy Rhinol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Int Forum Allergy Rhinol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos