Your browser doesn't support javascript.
loading
Development of a new effective African swine fever virus vaccine candidate by deletion of the H240R and MGF505-7R genes results in protective immunity against the Eurasia strain.
Li, Jiangnan; Song, Jie; Zhou, Shijun; Li, Shuai; Liu, Jia; Li, Tingting; Zhang, Zhaoxia; Zhang, Xianfeng; He, Xijun; Chen, Weiye; Zheng, Jun; Zhao, Dongming; Bu, Zhigao; Huang, Li; Weng, Changjiang.
Afiliação
  • Li J; Division of Fundamental Immunology, National African Swine Fever Para-reference Laboratory, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin, Heilongjiang, China.
  • Song J; Heilongjiang Provincial Key Laboratory of Veterinary Immunology , Harbin, Heilongjiang, China.
  • Zhou S; Division of Fundamental Immunology, National African Swine Fever Para-reference Laboratory, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin, Heilongjiang, China.
  • Li S; Heilongjiang Provincial Key Laboratory of Veterinary Immunology , Harbin, Heilongjiang, China.
  • Liu J; Division of Fundamental Immunology, National African Swine Fever Para-reference Laboratory, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin, Heilongjiang, China.
  • Li T; Heilongjiang Provincial Key Laboratory of Veterinary Immunology , Harbin, Heilongjiang, China.
  • Zhang Z; Division of Fundamental Immunology, National African Swine Fever Para-reference Laboratory, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin, Heilongjiang, China.
  • Zhang X; Heilongjiang Provincial Key Laboratory of Veterinary Immunology , Harbin, Heilongjiang, China.
  • He X; Division of Fundamental Immunology, National African Swine Fever Para-reference Laboratory, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin, Heilongjiang, China.
  • Chen W; Heilongjiang Provincial Key Laboratory of Veterinary Immunology , Harbin, Heilongjiang, China.
  • Zheng J; Division of Fundamental Immunology, National African Swine Fever Para-reference Laboratory, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin, Heilongjiang, China.
  • Zhao D; Heilongjiang Provincial Key Laboratory of Veterinary Immunology , Harbin, Heilongjiang, China.
  • Bu Z; Division of Fundamental Immunology, National African Swine Fever Para-reference Laboratory, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin, Heilongjiang, China.
  • Huang L; Heilongjiang Provincial Key Laboratory of Veterinary Immunology , Harbin, Heilongjiang, China.
  • Weng C; Division of Fundamental Immunology, National African Swine Fever Para-reference Laboratory, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin, Heilongjiang, China.
J Virol ; 97(10): e0070423, 2023 10 31.
Article em En | MEDLINE | ID: mdl-37768081
IMPORTANCE: African swine fever (ASF) caused by ASF virus (ASFV) is a highly contagious and acute hemorrhagic viral disease in domestic pigs. Until now, no effective commercial vaccine and antiviral drugs are available for ASF control. Here, we generated a new live-attenuated vaccine candidate (ASFV-ΔH240R-Δ7R) by deleting H240R and MGF505-7R genes from the highly pathogenic ASFV HLJ/18 genome. Piglets immunized with ASFV-ΔH240R-Δ7R were safe without any ASF-related signs and produced specific antibodies against p30. Challenged with a virulent ASFV HLJ/18, the piglets immunized with high-dose group (105 HAD50) exhibited 100% protection without clinical symptoms, showing that low levels of virus replication with no observed pathogenicity by postmortem and histological analysis. Overall, our results provided a new strategy by designing live-attenuated vaccine candidate, resulting in protection against ASFV infection.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vacinas Atenuadas / Vacinas Virais / Deleção de Genes / Vírus da Febre Suína Africana / Genes Virais Limite: Animals Idioma: En Revista: J Virol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vacinas Atenuadas / Vacinas Virais / Deleção de Genes / Vírus da Febre Suína Africana / Genes Virais Limite: Animals Idioma: En Revista: J Virol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos