CUBIC: A Versatile Cumate-Based Inducible CRISPRi System in Streptomyces.
ACS Synth Biol
; 12(10): 3143-3147, 2023 10 20.
Article
em En
| MEDLINE
| ID: mdl-37801665
ABSTRACT
Streptomyces, a genus of Gram-positive bacteria, is known as nature's medicine maker, producing a plethora of natural products that have huge benefits for human health, agriculture, and biotechnology. To take full advantage of this treasure trove of bioactive molecules, better genetic tools are required for the genetic engineering and synthetic biology of Streptomyces. We therefore developed CUBIC, a novel CUmate-Based Inducible CRISPR interference (CRISPRi) system that allows highly efficient and inducible gene knockdown in Streptomyces. Its broad application is shown by the specific and nondisruptive knockdown of genes involved in growth, development and antibiotic production in various Streptomyces species. To facilitate hyper-efficient plasmid construction, we adapted the Golden Gate assembly to achieve 100% cloning efficiency of the protospacers. We expect that the versatile plug-and-play CUBIC system will create new opportunities for research and innovation in the field of Streptomyces.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Streptomyces
Limite:
Humans
Idioma:
En
Revista:
ACS Synth Biol
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Holanda