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Experimental analysis of diverse actin-like proteins from various magnetotactic bacteria by functional expression in Magnetospirillum gryphiswaldense.
Awal, Ram Prasad; Müller, Frank D; Pfeiffer, Daniel; Monteil, Caroline L; Perrière, Guy; Lefèvre, Christopher T; Schüler, Dirk.
Afiliação
  • Awal RP; Department of Microbiology, Universitat Bayreuth , Bayreuth, Germany.
  • Müller FD; Department of Microbiology, Universitat Bayreuth , Bayreuth, Germany.
  • Pfeiffer D; Department of Microbiology, Universitat Bayreuth , Bayreuth, Germany.
  • Monteil CL; Aix-Marseille Université, CEA, CNRS, Institute of Biosciences and Biotechnologies of Aix-Marseille , Saint-Paul-lez-Durance, France.
  • Perrière G; Laboratoire de Biométrie et Biologie Evolutive, Université Claude Bernard-Lyon 1 , Villeurbanne, France.
  • Lefèvre CT; Aix-Marseille Université, CEA, CNRS, Institute of Biosciences and Biotechnologies of Aix-Marseille , Saint-Paul-lez-Durance, France.
  • Schüler D; Department of Microbiology, Universitat Bayreuth , Bayreuth, Germany.
mBio ; 14(5): e0164923, 2023 Oct 31.
Article em En | MEDLINE | ID: mdl-37823629
IMPORTANCE: To efficiently navigate within the geomagnetic field, magnetotactic bacteria (MTB) align their magnetosome organelles into chains, which are organized by the actin-like MamK protein. Although MamK is the most highly conserved magnetosome protein common to all MTB, its analysis has been confined to a small subgroup owing to the inaccessibility of most MTB. Our study takes advantage of a genetically tractable host where expression of diverse MamK orthologs together with a resurrected MamK LUCA and uncharacterized actin-like Mad28 proteins from deep-branching MTB resulted in gradual restoration of magnetosome chains in various mutants. Our results further indicate the existence of species-specific MamK interactors and shed light on the evolutionary relationships of one of the key proteins associated with bacterial magnetotaxis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Magnetospirillum / Magnetossomos Idioma: En Revista: MBio Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Alemanha País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Magnetospirillum / Magnetossomos Idioma: En Revista: MBio Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Alemanha País de publicação: Estados Unidos