Tuning Plant Promoters Using a Simple Split Luciferase Method to Assess Transcription Factor-DNA Interactions.
ACS Synth Biol
; 12(11): 3482-3486, 2023 11 17.
Article
em En
| MEDLINE
| ID: mdl-37856867
ABSTRACT
Sequence features, including the affinity of binding motifs for their cognate transcription factors, are important contributors to promoter behavior. The ability to predictably recode affinity enables the development of synthetic promoters with varying levels of response to known cellular signals. Here we describe a luminescence-based microplate assay for comparing the interactions of transcription factors with short DNA probes. We then demonstrate how these data can be used to design synthetic plant promoters of varying strengths that respond to the same transcription factor.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Regulação da Expressão Gênica
Idioma:
En
Revista:
ACS Synth Biol
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Reino Unido