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Enabling mRNA Therapeutics: Current Landscape and Challenges in Manufacturing.
Youssef, Maryam; Hitti, Cynthia; Puppin Chaves Fulber, Julia; Kamen, Amine A.
Afiliação
  • Youssef M; Department of Bioengineering, McGill University, Montreal, QC H3A 0G4, Canada.
  • Hitti C; Department of Bioengineering, McGill University, Montreal, QC H3A 0G4, Canada.
  • Puppin Chaves Fulber J; Department of Bioengineering, McGill University, Montreal, QC H3A 0G4, Canada.
  • Kamen AA; Department of Bioengineering, McGill University, Montreal, QC H3A 0G4, Canada.
Biomolecules ; 13(10)2023 10 09.
Article em En | MEDLINE | ID: mdl-37892179
ABSTRACT
Recent advances and discoveries in the structure and role of mRNA as well as novel lipid-based delivery modalities have enabled the advancement of mRNA therapeutics into the clinical trial space. The manufacturing of these products is relatively simple and eliminates many of the challenges associated with cell culture production of viral delivery systems for gene and cell therapy applications, allowing rapid production of mRNA for personalized treatments, cancer therapies, protein replacement and gene editing. The success of mRNA vaccines during the COVID-19 pandemic highlighted the immense potential of this technology as a vaccination platform, but there are still particular challenges to establish mRNA as a widespread therapeutic tool. Immunostimulatory byproducts can pose a barrier for chronic treatments and different production scales may need to be considered for these applications. Moreover, long-term storage of mRNA products is notoriously difficult. This review provides a detailed overview of the manufacturing steps for mRNA therapeutics, including sequence design, DNA template preparation, mRNA production and formulation, while identifying the challenges remaining in the dose requirements, long-term storage and immunotolerance of the product.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pandemias / COVID-19 Limite: Humans Idioma: En Revista: Biomolecules Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Canadá

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pandemias / COVID-19 Limite: Humans Idioma: En Revista: Biomolecules Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Canadá