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Robust and rapid partitioning in thermoplastic.
Quan, Phenix-Lan; Alvarez-Amador, Maria; Jiang, Yuhe; Sauzade, Martin; Brouzes, Eric.
Afiliação
  • Quan PL; Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY, 11794, USA. eric.brouzes@stonybrook.edu.
  • Alvarez-Amador M; Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY, 11794, USA. eric.brouzes@stonybrook.edu.
  • Jiang Y; Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY, 11794, USA. eric.brouzes@stonybrook.edu.
  • Sauzade M; Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY, 11794, USA. eric.brouzes@stonybrook.edu.
  • Brouzes E; Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY, 11794, USA. eric.brouzes@stonybrook.edu.
Analyst ; 149(1): 100-107, 2023 Dec 18.
Article em En | MEDLINE | ID: mdl-37982399
Partitioning is the core technology supporting digital assays. It divides a sample into thousands of individual reactors prior to amplification and absolute quantification of target molecules. Thermoplastics are attractive materials for large scale manufacturing, however they have been seldomly used for fabricating partitioning arrays. Patitioning in thermoplastic devices has proven difficult due to the challenge of efficiently displacing the air trapped in the nanoliter structures during priming of thousands of chambers. Here, we report the design of an array of chambers made of thermoplastics where the progression of the liquid-air interface is controlled by capillary effects. Our device performs robust partitioning over a wide range of pressures and can be actuated at low pressure by a simple micropipette. Our thermoplastic device lays the foundation to cost-effective and instrument-free partitioning platforms, which could be deployed in low-resource settings.

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Analyst Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Analyst Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Reino Unido